酵母提取物试剂盒

酵母提取物 – U-¹³C（ISO1 或 L-ISO1）和未标记（ISO1-UNL 和 L-ISO1-UNL） – 专为内部标准化（即基质掺入）定量而设计用于非靶向和靶向代谢组学的实验和质量控制评估。这些提取物中的化合物涵盖广泛的代谢类别（例如氨基酸和有机酸、磷酸糖、辅酶），这些代谢类别与各种生化途径（例如柠檬酸和乙醛酸循环、氨基酸和核苷酸代谢、磷酸戊糖）和细胞/分子相关过程（例如免疫系统、凝血、DNA 代谢）。这些代谢物已通过多种 LC-/GC-MS 方法进行了严格表征，并且在简单重构后可用于各种研究用途。

套件内容

• 干燥的酵母提取物为 U-¹³C 和/或未标记。

• 文件包（通过二维码提供）。包装内含用户手册，其中包含示例程序和 LC-MS 方法，供用户参考。

➤ 用于质谱分析的稳定同位素标准品
➤ 用于代谢研究的稳定同位素标记产品 
➤ 稳定同位素标记的混合物、套装和试剂盒
➤ 用于 MS 组学和 MS/MS 筛选的混合物、套件和试剂盒

应用说明
➤ 在 Agilent 6495 三重四极杆 LC/MS 上通过同位素稀释质谱对脆性代谢物进行绝对定量

代谢物和脂质酵母提取物

经常问的问题

酵母提取物来自生命周期的哪个部分？ 酵母（毕赤酵母，菌株 CBS 7435）处于指数生长期。在此阶段，细胞使用大部分可用底物进行繁殖。所用酵母菌株的倍增时间略小于3小时。通过验证发酵过程中的 OD 600 测量值可确保指数增长。提取物制备详述于PMID: 23086617.  

U-¹³C (98%) 中的“U”指的是什么？ “U”表示统一标记的化合物。例如，NADP⁺，其公式为 C₂₁H₂₉N₇O₁₇P₃ 在其 ¹³C 标记形式中具有 21 个 C。

溶解干代谢酵母提取物的推荐程序是什么？ 溶解 ISO1 和 ISO1-UNL 的推荐程序如下：

1. 在 2 mL 溶剂（例如水、50% 甲醇）中重构提取物。
2.用手用力摇晃，间歇性高速涡旋（至少 2 分钟）。
3. 20°C、4000 rcf 离心 5 分钟。
4. 然后可以将澄清的标准溶液稀释（1/10 v/v）直接使用或进一步制备用于校准和基质添加。

上面的资源部分显示了此过程的视频演示。

这些酵母提取物在哪些样品类型中进行了测量？这些提取物已应用于人体组织（例如血浆）和细胞（例如结肠癌），通过各种 LC-和 GC-MS 方法进行 QC 或定量分析。例如，ISO1 的用户手册（通过 QR 码随试剂盒发货提供）概述了三个利用同位素比分析进行绝对或相对代谢物定量的应用示例。

酵母提取物中常见的分析物有哪些？ 在常规、批次间测量中观察到的分析物或脂肪酸/脂质的列表在各自的章节中给出 稳定同位素标记的混合物、套装和试剂盒 目录。该列表不是有限的，因为其他分析物已通过替代方案进行了鉴定（例如，辅酶 As – 乙酰基、丙二酰基、丙酰基；1-磷酸葡萄糖；1-磷酸果糖）。请询问是否有其他感兴趣的分析物，我们将进行调查。

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