膜蛋白

膜蛋白占所有表达蛋白的20%至30%。它们在结构生物学和药物开发领域引起了极大的兴趣，因为它们占当今所有药物靶标的一半以上。膜蛋白具有疏水性外部，相对动态，并且以相对较低的水平产生。尽管这些因素给获得用于核磁共振分析的稳定制剂带来了困难，但在研究膜蛋白时，可以使用磷脂和去垢剂来确保适当的溶解和稳定性。尽管膜蛋白具有重要的功能重要性，但确定这些膜蛋白的结构比球状蛋白更具挑战性。幸运的是，溶液和固体核磁共振都有许多样品制备条件可供选择。

Cambridge Isotope Laboratories， Inc. 提供由FB试剂生产的氘代磷脂和去垢剂，用于基于NMR的膜蛋白科学研究。

Stable Isotopes for Biomolecular NMR

➤ Deuterated Phospholipids and Detergents from FB Reagents

Buffers, Reagents, Lipids and Detergents view all

常见问题

使用氘代磷脂有什么好处？ 使用氘代洗涤剂和磷脂可以简化¹H-NMR波谱，并更容易在多维中检测来自生物分子的信号 核磁共振实验。此外，它们允许将偶极弛豫效应降至最低，这反过来又导致某些系统中的信号增强。

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