Cambridge Isotope Laboratories, Inc. Partners with ISOtopic Solutions to Release the New Stable Isotope-Labeled and Unlabeled Crude Lipid Yeast Extracts

Andrew Percy (PhD), CIL’s MS 'Omics Product Manager and Senior Applications Scientist for Mass Spectrometry, states, “These analytes are broadly dispersed across a multitude of metabolic classes and pathways. This represents the largest panel of stable isotope-labeled standards commercially available in a single mix for targeted profiling and relative quantification of the lipidome.”

Andrew goes on to say, “A major benefit of the stable isotope-labeled lipid yeast extract is that the fatty acids and lipids are uniformly ¹³C-labeled at high isotopic enrichment. ¹³C labeling provides superior isotopic stability and analytical reliability as compared to ²H (or deuterium) labeling.” 

Gerrit Hermann (PhD), founder and CEO of ISOtopic Solutions, states, “Up to now, the lack of suitable internal standards has been one of the major limiting factors to achieve absolute quantitation in the field of lipidomics. The use of internal standard is a prerequisite to ensure high precision and accuracy, as well as to correct for errors introduced during sample preparation procedures. However, due to the high number and structural diversity of lipid molecules, it is almost impossible to ensure molecule-specific stable isotope-labeled internal standardization. This explains why class-specific internal standards have been used to date. This U-¹³C-labeled lipid yeast extract might enable these shortcomings to be overcome. In fact, these U-¹³C-labeled yeast cell extracts have already been widely used in microbiological metabolism studies, including targeted quantitative metabolite profiling.”

Overall, these lipid yeast extract mixes (CIL catalog nos. L-ISO1 and L-ISO1-UNL) are well suited for researchers performing MS lipidomics and integrative MS ‘omics (involving lipidomics). Both of these products are available exclusively through CIL.