A Roadmap for Interpreting 13C Metabolite Labeling Patterns from Cells
Buescher, J.M.; Antoniewicz, M.R.; Boros, L.G.; et al.
Looking to increase the data quality and output of your 13C tracer study? This paper reviews key aspects of experimental design and data interpretation when utilizing 13C-labeled tracers to interrogate cellular metabolism. Topics include: a comparison of metabolic steady state and isotopic steady state, labeling patterns of metabolites and cellular compartments, and labeling for steady state and dynamic studies.