MS-based Quantitative Plasma Proteomics

Percy, A.J.; Byrns, S.; Pennington, S.R.; et al.

This review article provides a retrospective and prospective five-year view of quantifying human plasma proteins for biomarker development in a clinical setting. The highlighted methods are categorized under a fit-for-purpose approach (i.e., from Tier 1 to Tier 3 for clinical and exploratory assays, respectively) and primarily utilize a bottom-up methodology with stable isotope-labeled standards (at the protein or peptide level) for quantification. Discussed are the challenges, requirements, and strategies for translating candidate protein biomarkers from research to the clinic.