Isotope Tracing in Health and Disease
Dong, W.; Rawat, E.S.; Stephanopoulos, G.; et al.
Stable isotope tracing is a powerful tool in contemporary metabolic research for dissecting metabolic pathways, distinguishing metabolite utilization, and quantifying metabolic flux. In this review, the utility and versatility of metabolic tracing with stable isotopes (e.g., 13C6 D-glucose, 13C5 L-glutamine) is discussed as it pertains to recent studies in human health and disease (e.g., cancer, neurodegeneration, diabetes). Insights into current challenges and future applications of in vivo, intercellular, and subcellular isotope tracing are also provided.