Proteomic Analysis of pRb Loss Highlights a Signature of Decreased Mitochondrial Oxidative Phosphorylation

Nicolay, B.N.; Danielian, P.S.; Kottakis, F.; et al.

Metabolic isotopic analysis (MIA) utilizes stable isotope-enriched substrates and mass spectrometry to study metabolism in living cells and tissue. Because isotope-labeled substrates make the determination of metabolic pathways and flux in living cells or tissue possible, MIA has become an integrated platform in oncology research. This application note presents an example of MIA for studying metabolism in mice and cultured human cells used in oncology research by summarizing research conducted by Nicolay et al. Previously these researchers used MIA to study the inactivation of the pRB tumor suppressor in the fly model.