LC-MS-based Quantification of Intact Proteins: Perspective for Clinical and Bioanalytical Applications

van den Broek, I.; van Dongen, W.D.

Mass spectrometry as a routine tool in the clinic is becoming more and more of a reality every day, presenting numerous opportunities in the field of proteomics. This review provides a comprehensive overview of existing LC-MS bottom-up proteomic techniques as well as the potential for future approaches using intact protein quantification. It includes a comparison between the two approaches, the limitations of both, and some of the hurdles that will need to be overcome for successful detection.