NeuCode Proteomics and Bap1 Regulation of Metabolism

Baughman, J.M.; Rose, C.M.; Kolumam, G.; et al.

Capitalizing on the merits of neutron encoding (NeuCode), Coon J et al. applied this metabolic-labeling technique to genetically engineered mice models to investigate mammalian biology in vivo. After preliminary experiments on the labeling efficiency and quantitative accuracy, three tissues (namely liver, pancreas, and spleen) were selected for further evaluation of Bap1, a deubiquitinating enzyme with cancer association. This research highlighted the utility of 4-plex NeuCode in mammals and revealed Bap1 to be a key regulator of metabolic homeostasis.