A Guide to 13C Metabolic Flux Analysis for the Cancer Biologist

Antoniewicz, M.R.

Here Maciek Antoniewicz reviews 13C metabolic flux analysis (MFA) for quantifying intracellular fluxes in cancer cell metabolism. Detailed are the fundamentals for experimental design, analytical measurement, and statistical analysis along with the merits and limitations of various tracer approaches.

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IsoTopics – December 2018

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13C-Labeled Yeast as Internal Standard for LC-MS/MS and LC High Resolution MS Based Amino Acid Quantification in Human Plasma
Glutamine-Derived 2-Hydroxyglutarate Is Associated with Disease Progression in Plasma Cell Malignancies
Hepatic Ketogenic Insufficiency Reprograms Hepatic Glycogen Metabolism and the Lipidome
A Targeted Metabolomics Approach for Clinical Diagnosis of Inborn Errors of Metabolism
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