As of June 12, 2018 our Privacy Policy has been updated. For individuals in the European Union, CIL uses cookies on this website. Please review the new privacy statement to see how. By continuing to use this website you agree to us using cookies in accordance with our privacy statement. Click here for the new privacy statement..OK

Corporate Overview

IsoTopics™ - May 2022

Temporal Quantitative Profiling of Newly Synthesized Proteins during Aß Accumulation

Ma,Y.; McClatchy, D.B.; Martínez-Bartolomé, S.; et al.

Alzheimer’s disease (AD) continues to be one of the more widespread neurodegenerative diseases, yet its pathogenesis remains unclear. A causative factor is believed to attribute from the accumulation of amyloid-β (Aβ) peptides in the brain. To identify the protein regulatory network and probe the Aβ peptide effects on the brain proteome, Yates et al. applied a refined method involving 13C4/15N2-labeled and unlabeled azidohomoalanine (AHA) to the brain extracts of variously aged mouse AD models. This metabolic AHA-labeling approach selectively enriches newly synthesized proteins (NSPs) through biotin-based click reactions enabling the sensitive quantitative of NSPs in mouse brain tissues. This MS proteomics study provided further insights into the mechanism of AD pathogenesis.

Read article

Stable Isotope Newsletters | Cambridge Isotope Laboratories
stable isotope, stable isotope labeled compounds, environmental contaminant standards
CIL has been ready to help with the analytical standards critical to the task of defining and resolving any major environmental contamination problems.