As of June 12, 2018 our Privacy Policy has been updated. For individuals in the European Union, CIL uses cookies on this website. Please review the new privacy statement to see how. By continuing to use this website you agree to us using cookies in accordance with our privacy statement. Click here for the new privacy statement..OK

Corporate Overview

IsoTopics™ – October 2017

Multiplexed MRM-Based Protein Quantitation Using Two Different Stable Isotope-Labeled Peptide Isotopologues for Calibration

LeBlanc, A.; Michaud, S.A.; Percy, A.J.; et al.
Toward enhanced depth and precision/accuracy of multiplexed protein quantitation, LeBlanc et al. developed a new calibration approach utilizing two variations of stable isotope-labeled tryptic peptides (referred to as SIS 1 and SIS 2). The SIS 1 peptides have a C-terminal 13C/15N-label, while SIS 2 has an internal 13C/15N-labeled residue in addition to a C-terminal label. Procedurally, external curves and QCs are prepared in a surrogate matrix using a constant amount of SIS 1 and a variable amount of SIS 2. This double-SIS-peptide approach overcomes matrix-induced interference from endogenous peptides and, compared to conventional quantitative approaches, provides superior figures of merit for improved method development and assay validation. 
Read article 

Stable Isotope Newsletters | Cambridge Isotope Laboratories
stable isotope, stable isotope labeled compounds, environmental contaminant standards
CIL has been ready to help with the analytical standards critical to the task of defining and resolving any major environmental contamination problems.