As of June 12, 2018 our Privacy Policy has been updated. For individuals in the European Union, CIL uses cookies on this website. Please review the new privacy statement to see how. By continuing to use this website you agree to us using cookies in accordance with our privacy statement. Click here for the new privacy statement..OK

Corporate Overview

IsoTopics™ – November 2022

Cardiac Myosin Filaments are Maintained by Stochastic Protein Replacement

Wood, N.B.; Kelly, C.M.; O’Leary, T.S.; et al.

Cardiac proteins are known to be intricately organized within filaments of muscle sarcomeres, but the mechanisms of protein turnover in these filaments remains a matter of debate. To examine the temporal and spatial dynamics of myosin replacement from the filament of mouse hearts, Previs and colleagues labeled proteins in vivo through metabolic incorporation of D3 L-leucine (via a mouse feed diet). A variety of analytical techniques, including MS-based bottom-up proteomics, were employed to quantify the mechanisms of newly synthesized protein synthesis in the heart. The results provide novel insights into protein turnover in cardiac muscle and the dynamic nature of their filamentous macromolecular assemblies.


Read article 


Stable Isotope Newsletters | Cambridge Isotope Laboratories
stable isotope, stable isotope labeled compounds, environmental contaminant standards
CIL has been ready to help with the analytical standards critical to the task of defining and resolving any major environmental contamination problems.