Relative Quantification and Higher-Order Model of Plasma Glycan Cancer Burden Ratio in Ovarian Cancer Case-Control Samples
Investigations into the regulation of the N-linked glycome by cancer and disease demands rigorous quantitative methods afforded by stable isotope labeling and identification given by the specificity of mass spectrometry. N-linked glycans present challenges to MS because of the complexity of their structures (MS/MS or MSn required), hydrophilicity, and low abundance. The Individuality Normalization when Labeling with Glycan Hydrazide Tags (INLIGHT®) strategy removes the burden of pre-enrichment, enhances ESI sensitivity, and allows accurate relative quantification. Applied to ovarian cancer research, these advantages of INLIGHT® facilitated the observation of low-abundant glycans, quantitative determination of highly significantly regulated compositions, and revealed new trends in glycan expression with cancer stage.
Abstract
Related Products:
Inlight Glycan Tagging Kits - Catalog No. GTK-1000
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