TMTpro: Design, synthesis, and initial evaluation of a proline-based isobaric 16-plex tandem mass tag reagent set
Thompson, A.; Wölmer, N.; Koncarevic, S.; et al.
Isobaric tagging has become an established tool in quantitative mass spectrometry for an array of proteomic-driven applications. Given the demands for increased multiplexing, new isobaric peptide tagging reagents were developed. These reagents, termed TMTpro™, utilize 13C/15N isobutylproline as reporters instead of the classical glycine-based tags used in alternate isobaric reagent sets, for increased multiplexing capacity. Demonstrated here is the synthesis and performance of the novel 16-plex TMTpro reagents for use in discovery and quantitative MS proteomics. These reagents provide high labeling efficiency and quantitative accuracy toward opportunities for expanded applications in MS ‘omics.
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- TMTpro: Design, synthesis, and initial evaluation of a proline-based isobaric 16-plex tandem mass tag reagent set
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