Noninvasive Liquid Diet Delivery Of Stable Isotopes Into Mouse Models For Deep Metabolic Network Tracing
Sun, R.C.; Fan, T.W.; Deng, P.; et al.
To help overcome the challenges associated with in vivo tracer studies of model animals, Fan and colleagues developed a liquid diet procedure to deliver 13C6-glucose into NSG mice. The administration is effective and non-invasive, enabling extensive metabolic tracing of a myriad of biochemical pathways, as quantified in tissue-based MS and NMR analysis. This developed approach was ultimately applied to patient-derived lung tumor xenografts as a function of mouse generations to track tumor-specific metabolic networks in NSG mice and compared to other tissue culture models to probe the effects of cancer cell metabolism on the tumor microenvironment.
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