As of June 12, 2018 our Privacy Policy has been updated. For individuals in the European Union, CIL uses cookies on this website. Please review the new privacy statement to see how. By continuing to use this website you agree to us using cookies in accordance with our privacy statement. Click here for the new privacy statement..OK

Products
Research Products
Amino Acids Bile Acids Biochemical Pathways Biological Standards Buffers and Reagents Carbohydrates CellFree Protein Expression Cell Growth Media Chemical Tagging Reagents Deuterated Reagents for ElectronicsDeuterated Reagents for PharmaceuticalsElements and Compounds Fatty Acids & Lipids Drug Standards Gases cGMP ProductsGlycans Key Metabolite Standards a-Keto Acids13C Metabolic Probes Metabolomics Mixes and Kits Metabolites & Substrates Methyl Labeling Kits & Amino Acids		 Metabolomics & Cellular 
MetabolismMicrobiological & Pyrogen Tested Mouse Express® Mouse Feed MRI/MRS Products MRM PeptiQuant™ Assay Kits MS/MS StandardsNMR Solvents and ConsumablesNucleic AcidsOrganic Acids & Conjugate Salts Protein StandardsProtein Reagents15N SaltsSILAC ReagentsSpirulinaSteroids and HormonesSynthetic IntermediatesVitaminsWater		 Environmental Contaminant Standards
Cyanotoxin Standards
Dioxin & Furan Individual Standards Dioxin & Furan Method Standards, Standard Mixtures, and Reference Materials PCB Standards & Standard MixturesFlame Retardants Dioxin/Furan Plus PCB Standard Mixtures Polycyclic Aromatic Hydrocarbon (PAH) Standards and Standard Mixtures Priority Pollutant Standard Mixtures Priority Pollutant, Endocrine Disruptor, and Chemical Contaminant Standards
Pesticides, Herbicides, and Metabolites Standards PPCPs Phthalate & Phthalate Metabolite Standards Chemical Weapon Metabolite Standards Per- and Polyfluoroalkyl Substances (PFAS)Stockholm Treaty Compounds

 

New Products

Corporate Overview

IsoTopics™ – July 2016

Stable-Isotope Labeling of Algal Biomass Carbohydrates | Cambridge Isotope Laboratories, Inc.

Given the growing interest in algal biomass in biological and biofuel applications, Antoniewicz M et al. developed a new analytical method to more conveniently quantify carbohydrates in C. vulgaris.  The method involved two-step HCl hydrolysis, chemical derivatization of monomer sugars by aldonitrile propionate, and GC-MS analysis. The use of 13C-labeled carbohydrates (e.g., glucose) enabled their composition and metabolic flux to be determined in C. vulgaris media grown autotrophically and heterotrophically.

Abstract

We have developed a method to measure carbohydrate composition and stable-isotope labeling in algal biomass using gas chromatography/mass spectrometry (GC/MS). The method consists of two-stage hydrochloric acid hydrolysis, followed by chemical derivatization of the released monomer sugars and quantification by GC/MS. Fully (13)C-labeled sugars are used as internal standards for composition analysis. This convenient, reliable, and accurate single-platform workflow offers advantages over existing methods and opens new opportunities to study carbohydrate metabolism of algae under autotrophic, mixotrophic, and heterotrophic conditions using metabolic flux analysis and isotopic tracers such as (2)H2O and (13)C-glucose. 

McConnell BO, Antoniewicz MR.

Read article 






View New Environmental Products

View All New Products

 

Articles

Stable Isotope Newsletters | Cambridge Isotope Laboratories
stable isotope, stable isotope labeled compounds, environmental contaminant standards
CIL has been ready to help with the analytical standards critical to the task of defining and resolving any major environmental contamination problems.