Stable-Isotope Labeling of Algal Biomass Carbohydrates | Cambridge Isotope Laboratories, Inc.
Given the growing interest in algal biomass in biological and biofuel applications, Antoniewicz M et al. developed a new analytical method to more conveniently quantify carbohydrates in C. vulgaris. The method involved two-step HCl hydrolysis, chemical derivatization of monomer sugars by aldonitrile propionate, and GC-MS analysis. The use of 13C-labeled carbohydrates (e.g., glucose) enabled their composition and metabolic flux to be determined in C. vulgaris media grown autotrophically and heterotrophically.
Abstract
McConnell BO, Antoniewicz MR.
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