IsoTopics™ – April 2017

SWATH-based Proteomics Identified Carbonic Anhydrase 2 as a Potential Diagnosis Biomarker for Nasopharyngeal Carcinoma

Luo, Y.; Mok, T.S.; Lin, X.; et al.

 

Given the prevalence of nasopharyngeal carcinoma (NPC) in Asian populations, there is an increasing need for diagnostic protein biomarkers. 

Serotonin Biosynthesis as a Predictive Marker of Serotonin Pharmacodynamics and Disease-Induced Dysregulation

Welford, R.W.; Vercauteren, M.; Trébaul, A., et al.

 

In a metabolomics-based tracer study, Welford and colleagues used ¹³C-labeled tryptophan as an oral tracer to measure/quantify the biosynthesis and metabolism of serotonin in rats. Here, the flux and pharmacokinetics of serotonin pathway metabolites were measured in various tissues by LC-MRM/MS. The pharmacodynamic effects of serotonin synthesis inhibition in vivo were also investigated. 

Comparative Analysis of INLIGHT™-labeled Enzymatically Depolymerized Heparin by Reverse-phase Chromatography and High-performance Mass Spectrometry

In an effort to overcome the analytical and technical challenges associated with glycosaminoglycan (GAG) analysis, Mangrum and colleagues applied the INLIGHT™ (individuality normalization when labeling with glycan hydrazide tags) labeling strategy to characterize and quantitatively analyze heparin digests (digHep).

13C Metabolic Flux Analysis of Acetate Conversion to Lipids by Yarrowia lipolytica

To better understand the metabolism of yeast Yarrowia lipolyticain the presence of volatile fatty acids, Stephanopoulos and colleagues performed a stationary ¹³C metabolic flux analysis using two isotopically labeled acetate tracers (¹³C₁ and ¹³C₂) as the feedstock.

Historical and Contemporary Stable Isotope Tracer Approaches to Studying Mammalian Protein Metabolism