IsoTopics™ – April 2016
Dear Researcher,
We are delighted to present the second edition of our newsletter, IsoTopicsTM! In this issue, we feature several articles published in the past few months that have had a significant impact on the mass spec community and cover a wide range of applications for stable isotopes. We hope they inspire further development in the MS field and collaboration among researchers from various disciplines.
In addition to the feeds we currently offer for SILAM applications, CIL is pleased to announce that Mouse Express® Neucode™ mouse feed will soon be available. This feed contains various combinations of labeled Lys isotopologues to metabolically label samples for multiplexed, absolute protein quantitation at high resolution. For more information, as well as a full listing of new products, please visit our featured product page or talk to one of our team members at an upcoming event or conference.
Please enjoy our latest edition of IsoTopicsTM and send any feedback our way!
Sincerely,
Your CIL Partners
Proteomics Product Manager
Deuterated Reagents & Intermediates Product Manager
China Sales - Research Products
Metabolic, MRI/MRS, Clinical
kateb@isotope.com
With the need for highly robust quantitative assays for phospho-signaling in mind, Paulovich, A. et al. developed an antibody-free, multiplex IMAC-MRM method with isotopically labeled peptide standards to quantify 107 phosphosites (93 proteins) in human cell lysates.
read moreDuchenne muscular dystrophy (DMD) is a genetic disorder characterized by progressive muscle degeneration.
read moreAnalyte identification is a major bottleneck in metabolomics studies.
read moreMeasurements of gluconeogenesis have been pivotal in elucidating the physiologic regulation of blood glucose homeostasis, as well as understanding the roles of glycogenolysis and gluconeogenesis in the pathophysiology of glucose metabolism in such conditions as obesity, metabolic acidosis, hypoglycemia, and diabetes.
read moreClinical laboratories are beginning to employ bottom-up MS-based methods with stable isotope-labeled standards for protein quantitation of biosamples, yet the procedures for development (from peptide selection to handling) and analysis are not well standardized.
read moreUsing MS-based proteomics for systems-wide profiling (instead of genomics and transcriptomics), Geiger, T. et al. employed FFPE filter-aided sample preparation (FASP) in combination with a super-SILAC approach to quantify >10,000 proteins (from >157,000 sequence-unique peptides) in clinical breast cancer tumor samples.
read moreArticles
- Immobilized Metal Affinity Chromatography Coupled to Multiple Reaction Monitoring Enables Reproducible Quantification of Phospho-signaling
- Discovery of Serum Protein Biomarkers in the mdx Mouse Model and Cross-Species Comparison to Duchenne Muscular Dystrophy Patients
- Isotopic Ratio Outlier Analysis of the S. cerevisiae Metabolome Using Accurate Mass Gas Chromatography/Time-of-Flight Mass
- Gluconeogenesis and Glycogenolysis Measurement Review
- Recommendations for Peptides Used In Mass Spectrometry Assays
- Proteomic Maps of Breast Cancer Subtypes
