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Metabolic Research

Metabolite Yeast Extracts

Metabolite Yeast Extracts Flyer


  • Metabolite Yeast Extracts Flyer

The yeast extracts -- U-13C (ISO1) and unlabeled (ISO1-UNL) -- are designed for internal standardization (i.e., matrix spike-in) quantitation experiments and for quality control evaluations in untargeted and targeted metabolomics. The compounds in these extracts span broad metabolic classes (e.g., amino and organic acids, sugar phosphates, coenzymes) that are linked to various biochemical pathways (e.g., citrate and glyoxylate cycle, amino acid and nucleotide metabolism, pentose phosphate) and cellular/molecular processes (e.g., immune system, blood coagulation, DNA metabolism). These metabolites have been rigorously characterized by several LC-/GC-MS methodologies and are amenable to a variety of research uses after simple reconstitution.  

Kit contents:
one dried down extract (either ISO1 or ISO1-UNL)
user manual (on USB)
 
 

Resources

 

For further information on the metabolite yeast extracts and its applications, please visit ISOtopic Solutions.

 

Frequently Asked Questions 

What does the U in U-13C (98%) refer to?

The U denotes a uniformly labeled compound.  For example, NADP+ with a formula C21H29N7O17P3 has 21 C in its 13C-labeled form.

What sample types have these yeast extracts been measured in?

The extracts have been applied to human tissue (e.g., plasma) and cells (e.g., colon cancer) for QC or quantitative analysis by a variety of LC- and GC-MS methods. Outlined in the user manual (supplied with the kit shipment on USB) of ISO1, for example, are three application examples that utilize isotope ratio analysis for absolute or relative metabolite quantification.

What are the commonly identified metabolites in the yeast extracts?

A tabulated list of metabolites observed across routine, batch-to-batch measurements is indicated on page 24 of our metabolomics catalog.  This list is not finite, as other metabolites have been identified with alternate protocols (e.g., coenzyme A’s – acetyl, malonyl, propionyl; glucose-1-phosphate; fructose-1-phosphate) or are subject to degradation (e.g., reduced nicotinamide adenine dinucleotide phosphate – NADPH).  Please inquire if other metabolites are of interest and we will investigate.

What are examples of other cofactors observed in the yeast extracts?

Additionally detected by HILIC-MS (positive ESI, Q Exactive HF) are NADPH (nicotinamide adenine dinucleotide phosphate), NMN (nicotinamide mononucleotide), and ADPR (adenosine diphosphate ribose).

What classes of metabolites are commonly observed?

These extracts enable the routine identification of a breadth of metabolites across a panel of classes.  This minimally includes amino acids and derivatives, organic acids and conjugates, sugar and sugar phosphates, vitamins, and co-enzymes. Additionally characterized are a collection of nucleobases, nucleosides, and nucleotides as well as lipids.

What part of the life cycle is the yeast extract from?

The yeast (Pichia Pastoris, strain CBS 7435) is in the exponential growth phase.  In this phase, the cell is using most of the available substrate to reproduce.  The doubling time for the yeast strain employed is a little less than 3 hours.  Exponential growth is ensured through verification of the OD 600 measurements during fermentation.  The extract preparation is detailed in PMID: 23086617.  

 

References

Hermann, G.; Schwaiger, M.; Volejnik, P.; et al. 2018. 13C-labelled yeast as internal standard for LC-MS/MS and LC high resolution MS-based amino acid quantification in human plasma. J Pharm Biomed Anal, 155, 329-334. PMID: 29704823

Guijas, C.; Montenegro-Burke, J.R.; Domingo-Almenara, X.; et al. 2018. METLIN: A technology platform for identifying knowns and unknowns. Anal Chem, 90(5), 3156-3164. PMID: 29381867

Si-Hung, L.; Causon, T.J.; Hann, S. 2017. Comparison of fully wettable RPLC stationary phases for LC-MS-based cellular metabolomics. Electrophoresis, 38(18), 2287-2295. PMID: 28691762

Schwaiger, M.; Rampler, E.; Hermann, G., et al. 2017. Anion-exchange chromatography coupled to high-resolution mass spectrometry: a powerful tool for merging targeted and non-targeted metabolomics. Anal Chem, 89(14), 7667-7674. PMID: 28581703

Ortmayr, K.; Hann, S.; Koellensperger, G. 2015. Complementing reversed-phase selectivity with porous graphitized carbon to increase the metabolome coverage in an on-line two-dimensional LC-MS setup for metabolomics. Analyst, 140(10), 3465-3473. PMID: 25824707

 

Krista Backiel

Krista Backiel

Marketing Manager and Metabolomics Manager

Krista Backiel is responsible for managing and promoting products that are utilized in Metabolomics and Clinical/Diagnostic MS. She spends a lot of her time developing new products to assist customers in their diverse research efforts.

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Andrew Percy, PhD

Andrew Percy, PhD

Senior Applications Chemist – Mass Spectrometry

Dr. Andrew Percy is the Senior Applications Chemist for Mass Spectrometry. His responsibilities minimally involve overseeing product development, identifying new product market opportunities, assisting in the analysis of products for MS ‘omics applications, and providing technical support to customers.

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