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Metabolic Research

TCA Cycle Mixtures

  • TCA Cycle Mixtures
The tricarboxylic acid (TCA) cycle, also known as the Krebs or citric acid cycle, plays an essential role in central carbon and energy metabolism. The study of its intermediates, and its ancillary compounds, has proven pivotal in not only understanding their impact on metabolism but also in profiling their function in oncogenesis, inflammation, and other pathologies.
To aid the analysis of TCA-associated compounds in metabolic studies, CIL is pleased to offer stable isotope-labeled and unlabeled TCA cycle mixes. These-dried down mixes comprise a collection of TCA cycle compounds as well as offshoot metabolites (see figure below for represented metabolites in red). 
Please refer to our TCA Cycle Metabolomics Mixtures flyer for the mix compositions and application examples.


Frequently Asked Questions 

What are the benefits of using multi-component mixtures?

The following lists a few of the top merits of using multi-component mixtures: eases user implementation, reduces development time and cost, enhances data quality, improves reproducibility, and improves confidence in the analytical results.

How many TCA intermediates and cycle offshoot metabolites comprise these mixes?

There are 6 TCA cycle intermediates and 7 offshoot metabolites. Please see the TCA Cycle Metabolomics Mixtures flyer for the metabolite panels. For a visual representation, the figure above illustrates the metabolites and pathways covered by the two TCA cycle mixes. Note that the applicable metabolites to the mixes are in red, while the compounds not included in the TCA mixes are in gray.

What is an example solvent for dissolving the TCA mixes?

The mix can be dissolved in LC-MS grade water. Reconstituting a given vial in 1 mL will yield an equimolar concentration of 100 µM.

How have the TCA mixes been measured?

Working solutions of these mixes (e.g., at 10 µM) have been measured by various analytical techniques. The mixes, for example, have been analyzed individually and in various ratio combinations by MS1 and MS2 on a HRAM mass spectrometer.

Can custom mixes or add-on vials be formulated?

Yes, we have the ability to customize. We would first review feasibility and then provide a quotation on your specific mix. To start this process, please provide the necessary details on this custom mix request form or contact your local sales representative.

Krista Backiel

Krista Backiel

Marketing Manager and Clinical MS Manager

Krista Backiel is responsible for managing and promoting products that are utilized in metabolomics and clinical/diagnostic MS. She spends a lot of her time developing new products to assist customers in their diverse research efforts.

Andrew Percy, PhD

Andrew Percy, PhD

Senior Applications Chemist – Mass Spectrometry

Dr. Andrew Percy is the Senior Applications Chemist for Mass Spectrometry and the MS ‘Omics Product Manager at CIL. His responsibilities minimally involve providing technical support, overseeing product development, identifying new product market opportunities, assisting in the analysis of product-related applications, and writing/reviewing marketing literature.