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Metabolic Research

Organic Acid Mixtures

Metabolomics Organic Acid Mixtures


  • Metabolomics Organic Acid Mixtures
  • App Note 47: Organic Acid Quantitation in Mouse Muscle by Ion Chromatography-Mass Spectrometry with Isotopically Labeled Standards
Organic acids (OAs) play essential roles in energy metabolism pathways (e.g., glycolysis, tricarboxylic acid cycle), with the short-chained OAs emerging as important regulators of host immune response and transcriptional regulation.
 
To aid qualification and quantification research in MS-based metabolomics, CIL is pleased to offer a collection of OA mixes in their stable isotope-labeled and unlabeled forms. These are available as a complete mix (comprised of 33 analytes; MSK-OA-1) and subset mixes (with 33 analytes spread across 7 subclasses e.g., 5 analytes in α-keto acid mix, MSK-OA1-1). All mixes are dried down, with 1 mL reconstitution yielding 250 µM equimolar concentration. Please refer to our Organic Acid Mixtures flyer for the metabolomic mix compositions.
 
 

Resources

  Metabolomics Organic Acid Mixtures

  Stable Isotope Standards for Mass Spectrometry

  Stable Isotope-Labeled Products for Metabolic Research

  Organic Acid Quantitation in Mouse Muscle by Ion Chromatography-Mass Spectrometry

 

Frequently Asked Questions 

What is an appropriate solvent for reconstituting the dried-down OA mixes?

Concentrated stock solutions of the labeled and unlabeled OA mixes can be prepared in aqueous solvents such as water or 0.1% formic acid (FA). Dilutions of working stocks for control and experimental sample use can then be prepared as necessary.

How many TCA cycle metabolites are present in the OA mixes?

There are 5 TCA-related metabolites in the complete mix. These metabolites are spread across 4 subset mixes – α-ketoglutarate in subset 1 (MSK-OA1), succinate and fumarate in subset 2 (MSK-OA2), malate in subset 4 (MSK-OA4), and citrate in subset 7 (MSK-OA7).

How have the mixes been measured?

All metabolites in the master mixes have been measured by HILIC-MS on an Orbitrap ID-X mass spectrometer (negative ESI).  Concentrated stocks of the metabolite subsets have effectively been measured by derivatization-based GC-MS.

If alternate organic acids are needed, can additional standards be provided?

Yes, we offer a breadth of individual stable isotope labeled organic acids beyond what is included in the mix. Please click here for the listing. In the event that your usage is routine, we can evaluate a custom mix or add-on vial to meet your needs. Refer to the Custom Mix Request form to complete your request for quotation or contact your local sales representative.
 

Krista Backiel

Krista Backiel

Marketing Manager and Metabolomics Manager

Krista Backiel is responsible for managing and promoting products that are utilized in metabolomics and clinical/diagnostic MS. She spends a lot of her time developing new products to assist customers in their diverse research efforts.

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Andrew Percy, PhD

Andrew Percy, PhD

Senior Applications Chemist – Mass Spectrometry

Dr. Andrew Percy is the Senior Applications Chemist for Mass Spectrometry. His responsibilities minimally involve overseeing product development, identifying new product market opportunities, assisting in the analysis of products for MS ‘omics applications, and providing technical support to customers.

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