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Metabolic Research


Stable Isotope Standards for Waters MetaboQuan-R™ Methods

  • Stable Isotope Standards for Waters MetaboQuan-R™ Methods
To expedite method implementation and decrease laboratory downtime/costs, Waters has developed a series of optimized UPLC-MRM/MS methods for a variety of biologically relevant analytes. The MetaboQuan-R™ methods enable the rapid (-R), multi-analyte analysis of different classes (e.g., acylcarnitines, amino acids, bile acids, free fatty acids) and pathways (e.g., tryptophan) of metabolites in human biosamples. The availability of these methods for a single instrument system enhances execution, throughput, and transferability toward targeted quantitation in bioanalytical- or biomedical-based metabolomics research.
To help facilitate the multi-omics MS analysis of targeted analytes in isotope dilution mass spectrometry (IDMS) applications, CIL is pleased to offer accompanying stable isotope-labeled standards. The internal standards (IS) available for these MetaboQuan-R methods are detailed in the method-specific flyers below (see Resources section). The IS can be supplied individually or as a set. Please inquire for details.
Method package contents for load-and-go operation:
Waters Quanpedia™ method file
Waters method-specific application note
CIL method-specific internal standards flyer 
Click here for further information on the MetaboQuan-R methods and its requisite hardware/software:

Frequently Asked Questions 

How many analytes are targeted in the MetaboQuan-R methods?

The panels consist of 20 acylcarnitines (12 IS and 8 surrogates), 29 amino acids (27 IS and 2 surrogates), 16 bile acids (15 IS and 1 surrogate), and 26 free fatty acids (15 IS and 11 surrogates). Also included in the method panels are 8 analytes (as IS) for tryptophan pathway analysis and 10 analytes (9 IS and 1 surrogate) for the TCA cycle analysis. Note, these analytes are analyzed in a multiplexed manner using a common platform/workflow, but with one metabolic class targeted per method (e.g., acylcarnitines run independently from amino acids).

On what LC-MS platform is sample processing achieved?

The metabolites are processed via UPLC-MS/MS. The LC-MS consists of an Acquity UPLC I-class system interfaced to a Xevo™ TQ-S micro or TQ-XS mass spectrometer. The column is typically a Cortecs™ T3 (2.1x30 mm, 2.7 µm particles). The instrument settings (e.g,. retention tims, ionization mode, MRM transitions) are all preprogrammed in the Quanpedia method file supplied in the Waters methods package (see Resources section for contents).

What are the method run times?

The LC gradients are method-dependent and are generally completed in <2.5 min.

What informatic tools are used in data analysis?

TargetLynx is used for data review and compound quantification.

Does CIL offer custom synthesis or custom mix services?

We will evaluate all requests provided that certain compound details are provided (see custom synthesis request or custom mix request form). Please complete and submit the form(s) on-line or contact your local sales representative.


Krista Backiel

Krista Backiel

Marketing Manager and Metabolomics Manager

Krista Backiel is responsible for managing and promoting products that are utilized in metabolomics and clinical/diagnostic MS. She spends a lot of her time developing new products to assist customers in their diverse research efforts.

Andrew Percy, PhD

Andrew Percy, PhD

Senior Applications Chemist – Mass Spectrometry

Dr. Andrew Percy is the Senior Applications Chemist for Mass Spectrometry and the MS ‘Omics Product Manager at CIL. His responsibilities minimally involve providing technical support, overseeing product development, identifying new product market opportunities, assisting in the analysis of product-related applications, and writing/reviewing marketing literature.