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Biomolecular NMR

Maltose Binding Protein (NEX-MBP)

NEX-MBP is a 44.9 kDa monomeric protein for which multiple sets of resonance assignments (BMRB database) and 3D structures (PDB database) are publicly available. This product is uniformly 2H,15N,13C-enriched with selective incorporation of protons into methyl groups of Ile-δ1, Leu-δ and Val-γ side chains. As nonuniform sampling (NUS) and other NMR techniques emerge to push the size limitations of NMR to new boundaries, large protein standards, such as NEX-MBP, will be required to test data-collection and processing strategies.

NEX-MBP sample formulations:

NEX-MBP1: Apo Conformation 0.5 mM 2 H,15N,13C and ILV methyl 1H,13C MBP in 10% D2O, 0.02% NaN3 , 20 mM sodium phosphate @ pH 7.2
NEX-MBP2: Closed Conformation 0.5 mM 2 H,15N,13C and ILV methyl 1 H,13C MBP with 3 mM maltotriose, 10% D2O, 0.02% NaN3 , 20 mM sodium phosphate @ pH 7.2
NEX-MBP3: Open Conformation 0.5 mM 2 H,15N,13C and ILV methyl 1 H,13C MBP with 2 mM β-cyclodextrin, 10% D2O, 0.02% NaN3 , 20 mM sodium phosphate @ pH 7.2 

 

E.coli Maltose Binding Protein (27-396), Apo Conformation

Catalog Label
NEX-MBP1-U unlabeled
NEX-MBP1-N (15N, 95%)
NEX-MBPI-CN-5 (13C, 5%; 15N, 95%)
(13C, 95%; 15N, 95%) 
(13C, 95%; D, 95%; 15N, 95%) 
(13C, 95%; D, 95%; 15N, 95%; 13CH3 -ILV) 
NEX-MBP1-ILVFY (13C, 95%; D, 95%; 15N, 95%; 13CH3 -ILVFY)

 

E.coli Maltose Binding Protein (27-396), Closed Conformation

Catalog No. Label
unlabeled
(15N, 95%)
(13C, 5%; 15N, 95%)
(13C, 95%; 15N, 95%)
(13C, 95%; D, 95%; 15N, 95%)
(13C, 95%; D, 95%; 15N, 95%; 13CH3-ILV)
NEX-MBP2-ILVFY
 (13C, 95%; D, 95%; 15N, 95%; 13CH3-ILVFY)


E.coli Maltose Binding Protein (27-396), Open Conformation

Catalog No. Label
NEX-MBP3-U
unlabeled
(15N, 95%)
NEX-MBP3-CN-5
(13C, 5%; 15N, 95%)
NEX-MBP3-CN
(13C, 95%; 15N, 95%)
NEX-MBP3-CDN
(13C, 95%; D, 95%; 15N, 95%)
(13C, 95%; D, 95%; 15N, 95%; 13CH3-ILV)
(13C, 95%; D, 95%; 15N, 95%; 13CH3-ILVFY)

 

 

 

Protein Sequence 
MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAH DRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEE IPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFL VDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKP FVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMEN AQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTRITK