Maltose Binding Protein (NEX-MBP)
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NEX-MBP is a 44.9 kDa monomeric protein for which multiple sets of resonance assignments (BMRB database) and 3D structures (PDB database) are publicly available. This product is uniformly 2H,15N,13C-enriched with selective incorporation of protons into methyl groups of Ile-δ1, Leu-δ and Val-γ side chains. As nonuniform sampling (NUS) and other NMR techniques emerge to push the size limitations of NMR to new boundaries, large protein standards, such as NEX-MBP, will be required to test data-collection and processing strategies.
NEX-MBP sample formulations:
NEX-MBP1: Apo conformation 0.5 mM 2 H,15N,13C and ILV methyl 1H,13C MBP in 10% D2O, 0.02% NaN3 , 20 mM sodium phosphate @ pH 7.2
NEX-MBP2: Closed conformation 0.5 mM 2 H,15N,13C and ILV methyl 1 H,13C MBP with 3 mM maltotriose, 10% D2O, 0.02% NaN3 , 20 mM sodium phosphate @ pH 7.2
NEX-MBP3: Open conformation 0.5 mM 2 H,15N,13C and ILV methyl 1 H,13C MBP with 2 mM β-cyclodextrin, 10% D2O, 0.02% NaN3 , 20 mM sodium phosphate @ pH 7.2
E.coli Maltose Binding Protein (27-396), Apo Conformation
Catalog | Label |
NEX-MBP1-U | unlabeled |
NEX-MBP1-N | (15N, 95%) |
NEX-MBPI-CN-5 | (13C, 5%; 15N, 95%) |
(13C, 95%; 15N, 95%)
|
|
(13C, 95%; D, 95%; 15N, 95%)
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|
(13C, 95%; D, 95%; 15N, 95%; 13CH3 -ILV)
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|
NEX-MBP1-ILVFY | (13C, 95%; D, 95%; 15N, 95%; 13CH3 -ILVFY) |
E.coli Maltose Binding Protein (27-396), Closed Conformation
Catalog No. | Label |
unlabeled
|
|
(15N, 95%)
|
|
(13C, 5%; 15N, 95%)
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|
(13C, 95%; 15N, 95%)
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|
(13C, 95%; D, 95%; 15N, 95%)
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|
(13C, 95%; D, 95%; 15N, 95%; 13CH3-ILV)
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|
NEX-MBP2-ILVFY |
(13C, 95%; D, 95%; 15N, 95%; 13CH3-ILVFY)
|
E.coli Maltose Binding Protein (27-396), Open Conformation
Catalog No. | Label |
NEX-MBP3-U
|
unlabeled |
(15N, 95%) | |
NEX-MBP3-CN-5 |
(13C, 5%; 15N, 95%)
|
NEX-MBP3-CN |
(13C, 95%; 15N, 95%)
|
NEX-MBP3-CDN |
(13C, 95%; D, 95%; 15N, 95%)
|
(13C, 95%; D, 95%; 15N, 95%; 13CH3-ILV)
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|
(13C, 95%; D, 95%; 15N, 95%; 13CH3-ILVFY)
|
Protein Sequence
MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAH DRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEE IPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFL VDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKP FVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMEN AQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTRITK