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Proteomics

Dimethyl Labeling

Stable Isotope Dimethyl Labeling (Application Note 38)

Shabaz Mohammed 
Departments of Chemistry and Biochemistry, University of Oxford, United Kingdom 
Biomolecular Mass Spectrometry and Proteomics Group, Utrecht University, Utrecht, The Netherlands 
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Submit or Request an Application Note
  • Stable Isotope Dimethyl Labeling (Application Note 38)
The dimethyl labeling technique uses a reagent mixture (i.e., cyanoborohydride and formaldehyde in their unlabeled and stable isotope-labeled forms) to tag primary amines (i.e., the N-terminus and the ε-amino group of lysine) in proteins or peptides. This is a fast, straightforward, and inexpensive approach to conduct 2- or 3-plex quantitative proteomic analyses of a variety of sample types (e.g., lysate, tissue). To facilitate such experiments (example provided in application note 38), Cambridge Isotope Laboratories, Inc. is pleased to offer a variety of reductive methylation reagents.
 

References 

Zhang, Y.; Xie, X.; Zhao, X.; Tian, F.; Lv, J.; Ying, W.;, Qian, X. 2018. Systems analysis of singly and multiply O-glycosylated peptides in the human serum glycoproteome via EThcD and HCD mass spectrometry. J Proteomics, 170, 14-27. PMID: 28970103

Lai, Z.W.; Bolm, L.; Fuellgraf, H.; et al. 2016. Characterization of various cell lines from different ampullary cancer subtypes and cancer associated fibroblast-mediated responses. BMC Cancer, 16, 195. PMID: 26951071

Tolonen, A.C.; Haas, W. 2014. Quantitative proteomics using reductive dimethylation for stable isotope labeling. J Vis Exp, (89). PMID: 25045933 

Weißer, J.; Lai, Z.W.; Bronsert, P.; et al. 2015. Quantitative proteomic analysis of formalin-fixed, paraffin-embedded clear cell renal cell carcinoma tissue using stable isotopic dimethylation of primary amines. BMC Genomics,16, 559.  PMID: 26220445

Munoz, J.; Low, T.Y.; Kok, Y.J.; Chin, A.; Frese, C.K.; Ding, V.; Choo, A.; Heck, A.J. 2011. The quantitative proteomes of human-induced pluripotent stem cells and embryonic stem cells. Mol Syst Biol, 7, 550. PMID: 22108792
 
Kevin Millis, PhD

Kevin Millis, PhD

Senior Scientist, Application Development Manager

Kevin Millis, PhD, is the Senior Scientist and Market Development Manager for all NMR and Mass Spectrometry product lines. Kevin is responsible for Technical Services both internally and externally for all CIL customers as well as being responsible for the application and market development for the CIL products.

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Reductive Methylation Reagents