Metabolic Research

Dimethyl Labeling

Stable Isotope Dimethyl Labeling (Application Note 38)

Shabaz Mohammed 
Departments of Chemistry and Biochemistry, University of Oxford, United Kingdom 
Biomolecular Mass Spectrometry and Proteomics Group, Utrecht University, Utrecht, The Netherlands 

  • Stable Isotope Dimethyl Labeling (Application Note 38)
The dimethyl labeling technique uses a reagent mixture (i.e., cyanoborohydride and formaldehyde in their unlabeled and stable isotope-labeled forms) to tag primary amines (i.e., the N-terminus and the ε-amino group of lysine) in proteins or peptides. This is a fast, straightforward, and inexpensive approach to conduct 2- or 3-plex quantitative proteomic analyses of a variety of sample types (e.g., lysate, tissue). To facilitate such experiments (example provided in application note 38), Cambridge Isotope Laboratories, Inc. is pleased to offer a variety of reductive methylation reagents.
 

References 

Lai, Z.W.; Bolm, L.; Fuellgraf, H.; et al. 2016. Characterization of various cell lines from different ampullary cancer subtypes and cancer associated fibroblast-mediated responses. BMC Cancer, 16, 195. PMID: 26951071

Weißer, J.; Lai, Z.W.; Bronsert, P.; et al. 2015. Quantitative proteomic analysis of formalin-fixed, paraffin-embedded clear cell renal cell carcinoma tissue using stable isotopic dimethylation of primary amines. BMC Genomics,16, 559.  PMID: 26220445

Munoz, J.; Low T.Y.; Kok,Y.J.; Chin, A.; Frese, C.K.; Ding, V.; Choo, A.; Heck, A.J. 2011. The quantitative proteomes of human-induced pluripotent stem cells and embryonic stem cells. Mol Syst Biol, 7, 550. PMID: 22108792
 
Tasha Agreste

Tasha Agreste

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Andrew Percy, PhD

Andrew Percy, PhD

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Kevin Millis, PhD

Kevin Millis, PhD

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