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Metabolic Research

Amino Acid Mixtures

Metabolomics Amino Acid Mixtures

For Identification and Quantification


  • Metabolomics Amino Acid Mixtures
CIL offers a number of amino acid mixtures for use in standardizing untargeted and/or targeted metabolite quantification experiments. These mixes comprise either canonical (i.e., essential and nonessential) or noncanonical (i.e., rare or unnatural) amino acids and are available in their 13C/15N (catalog nos. MSK-A2-1.2, MSK-CAA-1, MSK-NCAA-1) and unlabeled (catalog nos. MSK-A2-US-1.2, MSK-CAA-US-1, MSK-NCAA-US-1) form. Please refer to our Stable Isotope-Labeled Products for Metabolic Research catalog for the composition of each of these amino acid mixes.

In one example of its application, Figure 1 in PMID: 27609895 illustrates the relative quantification of these amino acids (from MSK-A2-1.2) in normal pancreatic tissues compared to pancreatic tumors. As these amino acids are present as intermediates in several metabolic pathways (e.g., glycolysis, citric acid cycle, pentose phosphate pathway), the use of these mixes in broader quantitative studies is possible (see example references below).                                                                                                                                                          

Resources

Metabolomics Amino Acid Mixtures
Stable isotope Standards for Mass Spectrometry
Stable Isotope-Labeled Products for Metabolic Research

 

Frequently Asked Questions 

In what form are the amino acid mixes?

The MSK-CAA mixes (labeled, MSK-CAA-1; unlabeled, MSK-CAA-US-1) and MSK-NCAA mixes (labeled, MSK-NCAA-1; unlabeled, MSK-NCAA-US-1) are dried down, while the MSK-A2 mixes (labeled, MSK-A2-1.2; unlabeled, MSK-A2-US-1.2) are supplied as a 1.2 mL solution (in 0.1 M HCl).

What are the metabolites and concentrations in the described amino acid mixes?

Please refer to page 29 in our Stable Isotope-Labeled Products for Metabolic Research catalog for the compositions.

In what matrices has MSK-A2-1.2 been applied?

This mix has been spiked (after dilution from its concentrated stock) into a range of human-based matrices (e.g., cell, tissue, plasma, and serum). An example set of references are listed below.

What is the shelf life of the amino acid mixes?

The recommended retest for all three MSK amino acid mixes is two years if stored in a refrigerator (-5 to 5°C, protected from light). Upon reconstitution, the amino acids in MSK-CAA mixes (labeled, MSK-CAA-1; unlabeled, MSK-CAA-US-1) and MSK-NCAA mixes (labeled, MSK-NCAA-1; unlabeled, MSK-NCAA-US-1) are stable in the refrigerator (-5 to 5°C, protected from light) for four weeks.

How do the MSK-CAA mixes differ from MSK-A2 mixes?

Relative to MSK-A2 mixes (labeled, MSK-A2-1.2; unlabeled, MSK-A2-US-1.2), MSK-CAA mixes (labeled, MSK-CAA-1; unlabeled, MSK-CAA-US-1) contain three additional amino acids (L-Asn, -Gln, and -Trp) and are dried down. This relates to the reconstitution condition and volume, which may impact the choice of sample preparative method and analysis technique.

Does CIL offer the ability to make custom mixes?

Yes, we do have the ability to customize. We would first review feasibility and then provide a quotation on your specific mix. To start this process, please provide the necessary details on this custom mix request form or contact your local sales representative. 

 

References

Lu, Y.; Wang, Y.; Liang, X.; et al. 2019. Serum amino acids in association with prevalent and incident type 2 diabetes in a Chinese population. Metabolites, 9(1). PMID: 30646552

Chen, W.W.; Freinkman, E.; Sabatini, D.M. 2017. Rapid immunopurification of mitochondria for metabolite profiling and absolute quantification of matrix metabolites. Nat Protoc12(10), 2215-2231. PMID: 29532801

Mehta, K.Y.; Wu, H.J.; Menon, S.S.; et al. 2017. Metabolomic biomarkers of pancreatic cancer: a meta-analysis study. Oncotarget, 8(40), 68899-68915. PMID: 28978166

Havelund, J.F.; Andersen, A.D.; Binzer, M.; et al. 2017. Changes in kynurenine pathway metabolism in Parkinson patients with L-DOPA-induced dyskinesia. J Neurochem, 142(5), 756-766. PMID: 28628213

Nemkov, T.; Hansen, K.C.; D'Alessandro, A. 2017. A three-minute method for high-throughput quantitative metabolomics and quantitative tracing experiments of central carbon and nitrogen pathways. Rapid Commun Mass Spectrom, 31(8), 663-673. PMID: 28195377

D'Alessandro, A.; Nemkov, T..; Yoshida, T.; et al. 2017. Citrate metabolism in red blood cells stored in additive solution-3. Transfusion, 57(2), 325-336. PMID: 27813142

Mayers, J.R.; Torrence, M.E.; Danai, L.V.; et al. 2016. Tissue of origin dictates branched-chain amino acid metabolism in mutant Kras-driven cancers. Science, 353(6304), 1161-1165. PMID: 27609895

Chen, W.W.; Freinkman, E.; Wang, T.; et al. 2016. Absolute Quantification of Matrix Metabolites Reveals the Dynamics of Mitochondrial Metabolism. Cell, 166(5), 1324-1337. PMID: 27565352

 

Krista Backiel

Krista Backiel

Marketing Manager and Metabolomics Manager

Krista Backiel is responsible for managing and promoting products that are utilized in metabolomics and clinical/diagnostic MS. She spends a lot of her time developing new products to assist customers in their diverse research efforts.

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Andrew Percy, PhD

Andrew Percy, PhD

Senior Applications Chemist – Mass Spectrometry

Dr. Andrew Percy is the Senior Applications Chemist for Mass Spectrometry. His responsibilities minimally involve overseeing product development, identifying new product market opportunities, assisting in the analysis of products for MS ‘omics applications, and providing technical support to customers.

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