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Metabolic Research

Amino Acid Mixtures

Stable Isotope-Labeled Products for Metabolomics


  • Stable Isotope-Labeled Products for Metabolomics

CIL offers an amino acid mixture for use in standardization in untargeted and/or targeted metabolite quantification experiments. This mix, which comprises 17 canonical amino acids, is available in its 13C/15N, (cat #MSK-A2-1.2) and unlabeled (cat #MSK-A2-US-1.2) form. Please refer to page 22 of our Stable Isotope-Labeled Products for Metabolomics catalog for the composition of these amino acid mixes. It has been implemented in a variety of MS-based applications. For example, Figure 1 on page 22 of this catalog illustrates the relative quantification of these amino acids (from MSK-A2-1.2) in normal pancreatic tissues compared to pancreatic tumors (Mayers, JR, et al., PMID: 27609895). As these amino acids are present as intermediates in several metabolic pathways (e.g., glycolysis, citric acid cycle, pentose phosphate pathway), the use of this mix in broader quantitative studies is possible (see example references below).

                                                                                                                                                               

Resources

Stable isotope Standards for Mass Spectrometry
Stable Isotope-Labeled Products for Metabolomics

 

Frequently Asked Questions 

In what form is the MSK-A2-1.2 mixture?

This mix is supplied as a 1.2 mL solution (in 0.1 M HCl).

What are the metabolites and concentrations in the MSK-A2-1.2 mixture?

The 17 13C/15N amino acids are tabulated in the metabolomics catalog (see page 22). The concentrations are 2.5 mM (exception being L-cystine at 1.2 mM) and reflect those used in NIST’s SRM 2389a unlabeled amino acid mix. The tolerance is ±10% for all compounds.

In what matrices has MSK-A2-1.2 been applied?

This mix has been spiked (after dilution from its concentrated stock) into a range of human-based matrices (e.g., cell, tissue, plasma, and serum). An example set of references are listed below.

What is the stability of the MSK-A2-1.2?

The mix is stable in a refrigerator (-5 to 5°C, protected from light) for a minimum of 2 years.

Does CIL offer the ability to make custom mixes?

Yes, we do have the ability to customize. We would first review feasibility and then provide a quotation on your specific mix. To start this process, please provide the necessary details on this custom mix request form or contact your local sales representative. 

 

References

Lu, Y.; Wang, Y.; Liang, X.; et al. 2019. Serum amino acids in association with prevalent and incident type 2 diabetes in a Chinese population. Metabolites, 9(1). PMID: 30646552

Chen, W.W.; Freinkman, E.; Sabatini, D.M. 2017. Rapid immunopurification of mitochondria for metabolite profiling and absolute quantification of matrix metabolites. Nat Protoc12(10), 2215-2231. PMID: 29532801

Mehta, K.Y.; Wu, H.J.; Menon, S.S.; et al. 2017. Metabolomic biomarkers of pancreatic cancer: a meta-analysis study. Oncotarget, 8(40), 68899-68915. PMID: 28978166

Havelund, J.F.; Andersen, A.D.; Binzer, M.; et al. 2017. Changes in kynurenine pathway metabolism in Parkinson patients with L-DOPA-induced dyskinesia. J Neurochem, 142(5), 756-766. PMID: 28628213

Nemkov, T.; Hansen, K.C.; D'Alessandro, A. 2017. A three-minute method for high-throughput quantitative metabolomics and quantitative tracing experiments of central carbon and nitrogen pathways. Rapid Commun Mass Spectrom, 31(8), 663-673. PMID: 28195377

D'Alessandro, A.; Nemkov, T..; Yoshida, T.; et al. 2017. Citrate metabolism in red blood cells stored in additive solution-3. Transfusion, 57(2), 325-336. PMID: 27813142

Mayers, J.R.; Torrence, M.E.; Danai, L.V.; et al. 2016. Tissue of origin dictates branched-chain amino acid metabolism in mutant Kras-driven cancers. Science, 353(6304), 1161-1165. PMID: 27609895

Chen, W.W.; Freinkman, E.; Wang, T.; et al. 2016. Absolute Quantification of Matrix Metabolites Reveals the Dynamics of Mitochondrial Metabolism. Cell, 166(5), 1324-1337. PMID: 27565352

 

Krista Backiel

Krista Backiel

Marketing Manager and Metabolomics Manager

Krista Backiel is responsible for managing and promoting products that are utilized in metabolomics and clinical/diagnostic MS. She spends a lot of her time developing new products to assist customers in their diverse research efforts.

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Andrew Percy, PhD

Andrew Percy, PhD

Senior Applications Chemist – Mass Spectrometry

Dr. Andrew Percy is the Senior Applications Chemist for Mass Spectrometry. His responsibilities minimally involve overseeing product development, identifying new product market opportunities, assisting in the analysis of products for MS ‘omics applications, and providing technical support to customers.

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