Amino Acid Mixtures
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Metabolomics Amino Acid Mixtures
For Identification and Quantification
- Metabolomics Amino Acid Mixtures
In one example of its application, Figure S3 in PMID: 27609895 illustrates the relative quantification of these amino acids (from MSK-A2-1.2) in normal pancreatic tissues compared to pancreatic tumors. As these amino acids are present as intermediates in several metabolic pathways (e.g., glycolysis, citric acid cycle, pentose phosphate pathway), the use of these mixes in broader quantitative studies is possible (see example references below).
Resources
Metabolomics Amino Acid Mixtures
Sets, Mixes, and Kits for MS 'Omics and MS/MS Screening
Stable Isotope Standards for Mass Spectrometry
Frequently Asked Questions
In what form are the amino acid mixes?
The MSK-CAA mixes (labeled, MSK-CAA-1; unlabeled, MSK-CAA-US-1) and MSK-NCAA mixes (labeled, MSK-NCAA-1; unlabeled, MSK-NCAA-US-1) are dried down, while the MSK-A2 mixes (labeled, MSK-A2-1.2; unlabeled, MSK-A2-US-1.2) are supplied as a 1.2 mL solution (in 0.1 M HCl).
What are the metabolites and concentrations in the described amino acid mixes?
Please refer to page 29 in our Stable Isotope-Labeled Products for Metabolic Research catalog for the compositions. Note that the metabolites and concentrations in a given unlabeled mix match their labeled counterpart (e.g., MSK-CAA-US-1 is equivalent to MSK-CAA-1, with the only difference being the stable isotope labeling).
In what matrices has MSK-A2-1.2 been applied?
This mix has been spiked (after dilution from its concentrated stock) into a range of human-based matrices (e.g., cell, tissue, plasma, and serum). An example set of references are listed below.
What is the shelf life of the amino acid mixes?
The recommended retest for all three MSK amino acid mixes is two years if stored in a refrigerator (-5 to 5°C, protected from light). Upon reconstitution, the amino acids in MSK-CAA mixes (labeled, MSK-CAA-1; unlabeled, MSK-CAA-US-1) and MSK-NCAA mixes (labeled, MSK-NCAA-1; unlabeled, MSK-NCAA-US-1) are stable in the refrigerator (-5 to 5°C, protected from light) for four weeks.
How do the MSK-CAA mixes differ from MSK-A2 mixes?
Relative to MSK-A2 mixes (labeled, MSK-A2-1.2; unlabeled, MSK-A2-US-1.2), MSK-CAA mixes (labeled, MSK-CAA-1; unlabeled, MSK-CAA-US-1) contain three additional amino acids (L-Asn, -Gln, and -Trp) and are dried down. This relates to the reconstitution condition and volume, which may impact the choice of sample preparative method and analysis technique.
What is an appropriate solvent for solubilizing the MSK-CAA and -NCAA mixes?
Suitable examples of dissolution solvents are water or water/methanol. It is imperative that the CAA mix is not reconstituted in 0.1 M HCl, as L-Asn and L-Gln are known to be unstable in acid.
Does CIL offer the ability to make custom mixes?
Yes, we do have the ability to customize. We would first review feasibility and then provide a quotation on your specific mix. To start this process, please provide the necessary details on this custom mix request form or contact your local sales representative.
References
Fuenzalida, K.; Leal-Witt, M.J.; Guerrero, P.; et al. 2021. NTBC treatment monitoring in Chilean patients with tyrosinemia type 1 and its association with biochemical parameters and liver biomarkers. J Clin Med, 10(24), 5832-5845. PMID: 34945128
Lim, E.W.; Handzlik, M.K.; Trefts, E.; et al. 2021. Progressive alterations in amino acid and lipid metabolism correlate with peripheral neuropathy in PolgD257A mice. Sci Adv, 7(42), eabj4077. PMID: 34652935
Gebert, N.; Rahman, S.; Lewis C.A.; et al. 2021. Identifying cell-type-specific metabolic signatures using transcriptome and proteome analyses. Curr Protoc, 1(9), e245. PMID: 34516047
Gauthier-Coles, G.; Vennitti, J.; Zhang, Z.; et al. 2021. Quantitative modelling of amino acid transport and homeostasis in mammalian cells. Nat Commun, 12(1), 5282-5300. PMID: 34489418
Gao, J.; Chung, T-S. 2021. Membranes made from nonsolvent-thermally induced phase separation (N-TIPS) for decellularization of blood in dry plasma spot (DPS) applications. Chem Eng Sci, 229. doi.org/10.1016/j.ces.2020.116010.
Liu, Y.; Zhao, Y.; Shukha, Y.; et al. 2021. Dysregulated oxalate metabolism is a driver and therapeutic target in atherosclerosis. Cell Rep, 36(4), 109420-109457. PMID: 34320345
Kimhofer, T.; Lodge, S.; Whiley, L.; et al. 2020. Integrative modeling of quantitative plasma lipoprotein, metabolic, and amino acid data reveals a multiorgan pathological signature of SARS-CoV-2 infection. J Proteome Res, 19(11), 4442-4454. PMID: 32806897
van Gastel, N.; Spinelli, J.B.; Sharda, A.; et al. 2020. Induction of a timed metabolic collapse to overcome cancer chemoresistance. Cell Metab, 32(3), 391-403. PMID: 32763164
Røst, L.M.; Brekke Thorfinnsdottir, L.; Kumar, K.; et al. 2020. Absolute quantification of the central carbon metabolome in eight commonly applied prokaryotic and eukaryotic model systems. Metabolites, 10(2), 74. PMID: 32093075
Alcock, R.D.; Shaw, G.C.; Tee, N.; et al. 2019. Plasma amino acid concentrations after the ingestion of dairy and collagen proteins, in healthy active males. Front Nutr, 6, 163. PMID: 31681789
Sullivan, M.R; Danai, L.V.; Lewis, C.A.; et al. 2019. Quantification of microenvironmental metabolites in murine cancers reveals determinants of tumor nutrient availability. Elife, 8, e44235. PMID: 30990168
Lu, Y.; Wang, Y.; Liang, X.; et al. 2019. Serum amino acids in association with prevalent and incident type 2 diabetes in a Chinese population. Metabolites, 9(1). PMID: 30646552
Haines, N.R.; Manoharan, N.; Olson, J.L.; et al. 2018. Metabolomics analysis of human vitreous in diabetic retinopathy and rhegmatogenous retinal detachment. J Proteome Res, 17(7), 2421-2427. PMID: 29877085
Chen, W.W.; Freinkman, E.; Sabatini, D.M. 2017. Rapid immunopurification of mitochondria for metabolite profiling and absolute quantification of matrix metabolites. Nat Protoc, 12(10), 2215-2231. PMID: 29532801
Pretorius, C.J.; McWhinney, B.C.; Sipinkoski, B.; et al. 2018. Rapid amino acid quantitation with pre-column derivatization; ultra-performance reverse phase liquid chromatography and single quadrupole mass spectrometry. Clin Chim Acta, 478, 132-139. PMID: 29274329
Mehta, K.Y.; Wu, H.J.; Menon, S.S.; et al. 2017. Metabolomic biomarkers of pancreatic cancer: a meta-analysis study. Oncotarget, 8(40), 68899-68915. PMID: 28978166
Havelund, J.F.; Andersen, A.D.; Binzer, M.; et al. 2017. Changes in kynurenine pathway metabolism in Parkinson patients with L-DOPA-induced dyskinesia. J Neurochem, 142(5), 756-766. PMID: 28628213
Nemkov, T.; Hansen, K.C.; D'Alessandro, A. 2017. A three-minute method for high-throughput quantitative metabolomics and quantitative tracing experiments of central carbon and nitrogen pathways. Rapid Commun Mass Spectrom, 31(8), 663-673. PMID: 28195377
D'Alessandro, A.; Nemkov, T..; Yoshida, T.; et al. 2017. Citrate metabolism in red blood cells stored in additive solution-3. Transfusion, 57(2), 325-336. PMID: 27813142
Mayers, J.R.; Torrence, M.E.; Danai, L.V.; et al. 2016. Tissue of origin dictates branched-chain amino acid metabolism in mutant Kras-driven cancers. Science, 353(6304), 1161-1165. PMID: 27609895
Chen, W.W.; Freinkman, E.; Wang, T.; et al. 2016. Absolute quantification of matrix metabolites reveals the dynamics of mitochondrial metabolism. Cell, 166(5), 1324-1337. PMID: 27565352
Krista Backiel
Marketing Manager and Metabolomics Manager
Krista Backiel is responsible for managing and promoting products that are utilized in metabolomics and clinical/diagnostic MS. She spends a lot of her time developing new products to assist customers in their diverse research efforts.
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Andrew Percy, PhD
Senior Applications Chemist – Mass Spectrometry
Dr. Andrew Percy is the Senior Applications Chemist for Mass Spectrometry and the MS ‘Omics Product Manager at CIL. His responsibilities minimally involve providing technical support, overseeing product development, identifying new product market opportunities, assisting in the analysis of product-related applications, and writing/reviewing marketing literature.
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