Metabolism

Analysis of Whole-Body Branched-Chain Amino Acid Metabolism in Mice Utilizing 20% Leucine ¹³C₆ and 20% Valine ¹³C₅ Mouse Feed (Application Note 43)

Jared R. Mayers, Margaret E. Torrence, Brian P. Fiske, Shawn M. Davidson, and Matthew G. Vander Heiden

Koch Institute for Integrative Cancer Research and Department of Biology Massachusetts Institute of Technology, Cambridge, MA USA

download

Submit an Application Note

Analysis of Whole-Body Branched-Chain Amino Acid Metabolism in Mice Utilizing 20% Leucine ¹³C₆ and 20% Valine ¹³C₅ Mouse Feed (Application Note 43)
The Impact of Stable Isotope Tracers on Metabolic Research
Cellular Metabolism and Metabolomics
Stable Isotopes in Drug Development and Personalized Medicine

Researchers employ stable isotope techniques to study a wide variety of metabolic disorders and diseases including Alzheimer’s, Parkinson’s, cancer, diabetes, and obesity. Isotopes are most commonly used in metabolism research as tracers to quantify biochemical or metabolic reactions in vivo. They can be used to study metabolic pathways, to determine biomarkers, to test the effects of a drug, and to develop metabolic profiles of biological systems in a particular state.

Product Grades
CIL provides additional testing to many of our products as a service to our customers. The following documents describe the nature of these products, the different levels of control applied to them, and the Enhanced Technical Data Package that is available for some products.

Resources

Cancer Metabolism and Related Research

Amino Acid Indicators and Protein Turnover

Research Use Statement

Product Quality Designation Chart

Enhanced Data Package

Frequently Asked Questions

When are CIL’s -MPT products tested for microbiological content?

They are tested in the bulk form at release. Subsequent aliquots are not retested and guaranteed upon receipt of order. Microbiological testing does not imply suitability for any intended use.

What organisms does the microbiological testing apply to?

The –MPT products are tested for S. aureus, P. aeruginosa, E. coli, Salmonella sp., aerobic bacteria, yeast, mold, and for bacterial endotoxins.

What is the limit for microbiological testing?

For most products, the limit is <10 cfu/g for aerobic bacteria, yeast, and mold. These products also “Pass” for S. aureus, P. aeruginosa, E. coli, Salmonella sp.

What is the limit for endotoxin testing (LAL)?

Most products have an LAL specification of <0.25 EU/mg, but some can be different (<0.125, <0.03, <0.01, etc). The actual LAL results are reported on the lot-specific CoA.

Can CIL perform additional tests on research grade (-0 products) or microbiological tested material (-MPT)?

Yes, CIL has the capabilities to perform additional testing on most products. This should be reviewed and quoted prior to order. An additional fee may apply.

Does CIL offer products for clinical trials?

Yes, CIL can produce cGMP grade material that is suitable for clinical trials. Please contact us to discuss your project.

What algal strain is used to create Mixed Triglycerides products?

Agmenelum quadriplicatum

What algal strain is used to create Mixed Fatty Acids products?

Agmenelum quadriplicatum

What algal strain is used to create the Amino Acid Mixes product?

Agmenelum quadriplicatum

What algal strain is the Whole Algal Cells product?

Agmenelum quadriplicatum

References

Hermans, Wesley JH; et al. 2021. Insects are a viable protein source for human consumption: from insect protein digestion to postprandial muscle protein synthesis in vivo in humans: a double-blind randomized trial,Am J Clin Nutr 114(3):934-944. PMID: 34020450

Roswell Shelhamer

Market Development Manager, Metabolic, MRI/MRS, Clinical

Ros Shelhamer joined Cambridge Isotope Laboratories, Inc. in 2014 as the Business Development Manager. His focus is developing new business opportunities for CIL within the MRS/MRI, Metabolomics and GMP product lines.