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MS/MS Standards

MS/MS Products

MS/MS Screening Mixtures and Standards

  • MS/MS Screening Mixtures and Standards
  • The Use of Stable Isotope-Enriched Standards as a Key Component of the MS/MS Analysis of Metabolites Extracted from Dried Blood Spots
Details and applications of our offerings (both sets and individuals) for MS/MS screening and tuning are noted in the resources and referencs below. In particular, the MS/MS Screening Standards catalog lists the composition details and reconstitution recommendations (for concentrated and working stocks), but also the usage specifications (i.e., storage and recommended retest date before and after reconstitution) for our reference standard sets. The sets contain a collection of stable isotope-labeled standards (e.g., 12 amino acids in NSK-A) and are available in a 10-vial set or as single vials. CIL also has the ability to customize standards or mixes to meet your specific needs. Please inquire for details.

Frequently Asked Questions 

What is the recommended procedure for solubilizing NSK-A and -B?

The lyophilized amino acid mix in NSK-A (and NSK-A1) should  be dissolved in 1 mL of 50:50 purified water:methanol and the carnitine/acylcarnitines in NSK-B (and NSK-B-G1) in 1 mL of  purified methanol. Following the solvent addition, each vial should be vortexed manually for 1 min then auto-vortexed for 30 min (or longer until fully solubilized).

Are the component chemical purities considered in the NSK product formulations?

Yes, the chemical purity of each component (as stated on the CoA) is reviewed at time of formulation and the gravimetry is adjusted as necessary to compensate for chemical purity.

What concentration should I use on the CoA to represent the standard in use – gravimetric target concentration, concentration by gravimetry, or analyzed concentration?

The concentrations presented in the “Concentration by Gravimetry” column of the CoA should be used to represent the actual concentration of each component after reconstitution.


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Young, A.; Hendricks, J.; Foreman, D.; et al. 2020. Development of dried blood spot quality control materials for adenosine deaminase severe combined immunodeficiency and an LC-MS/MS method for their characterization. Clin Mass Spec, 17, 4-11. PMID 33851028
Staretz-Chacham, O.; Daas, S.; Ulanovsky, I.; et al. 2021. The role of orotic acid measurement in routine newborn screening for urea cycle disorders. J Inherit Metab Dis, 44(3), 606-617. PMID: 33190319
Lai, F.; Srinivasan, S.; Wiley, V. 2020. Evaluation of a two-tier screening pathway for congenital adrenal hyperplasia in the New South Wales Newborn Screening Programme. Int J Neonatal Screen, 6(3), 63. PMID: 33117905
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Brennenstuhl, H.; Kohlmüller, D.; Gramer, G.; et al. 2020. High throughput newborn screening for aromatic ʟ-amino-acid decarboxylase deficiency by analysis of concentrations of 3-O-methyldopa from dried blood spots. J Inherit Metab Dis, 43(3), 602-610. PMID: 31849064
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Coene, K.L.M.; Kluijtmans, L.A.J.; van der Heeft, E.; et al. 2018. Next-generation metabolic screening: targeted and untargeted metabolomics for the diagnosis of inborn errors of metabolism in individual patients. J Inherit Metab Dis, 41(3), 337-353. PMID: 29453510
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Céspedes, N., Valencia, A., Echeverry, C.A., et al. 2017. Reference values of amino acids, acylcarnitines and succinylacetone by tandem mass spectrometry for use in newborn screening in southwest Colombia. Colomb Med, 48(3), 113-119. PMID: 29213153
de Sain-van der Velden, M.G.M.; van der Ham, M.; Gerrits, J.; et al. 2017. Quantification of metabolites in dried blood spots by direct infusion high resolution mass spectrometry. Anal Chim Acta, 979, 45-50. PMID: 28599708
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Krista Backiel

Krista Backiel

Marketing Manager and Metabolomics Manager

Krista Backiel is responsible for managing and promoting products that are utilized in metabolomics and clinical/diagnostic MS. She spends a lot of her time developing new products to assist customers in their diverse research efforts.

Andrew Percy, PhD

Andrew Percy, PhD

Senior Applications Chemist – Mass Spectrometry

Dr. Andrew Percy is the Senior Applications Chemist for Mass Spectrometry and the MS ‘Omics Product Manager at CIL. His responsibilities minimally involve providing technical support, overseeing product development, identifying new product market opportunities, assisting in the analysis of product-related applications, and writing/reviewing marketing literature.


Related Products

Amino Acid Standards

Carnitine/Acylcarnitine Standards

Organic Acid Standards

Steroid and Hormone Standards

Substrates & Internal Standards

Other Standards

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To view information on our CE mark* products click here. Their instructions for use can be obtained here.