Glycan Standards

Glycan Standards for Microarrays and the Identification/Quantification of Glycans

Glycan Standards for Microarrays and the Identification/Quantification of Glycans
Metabolic Incorporation of Stable Isotope Labels into Glycans (Application Note 23)

Glycan Standards

The glycan standards available are in ¹³C₆-labeled and unlabeled forms. These encompass a broad range of categories (e.g., N-linked, O-linked, and fucosylated) and have been highly characterized using a number of analytical techniques (e.g., ¹H NMR and ESI-MS). The standards are supplied as purified powders in 500 pmol quantities (alternate quantities available upon request) and can be used for a variety of applications (e.g., health and disease assessment).

Common Symbol Nomenclature for Unlabeled Monosaccharides:

Note: Labeled monosaccharide symbols (e.g.,¹³C₆ galactose) contain a black asterisk.

Resources

Glycan Standards and Glycan Quantitation Kit

Glycan Standards for Microarrays and the Identification/Quantifcation of Glycans

Stable Isotope Standards for Mass Spectrometry

Frequently Asked Questions

What is the symbol nomenclature for denoting glycans in chemical structures?

The symbols adhere to the modified IUPAC nomenclature, as recommended by the glycomics consortium network. This defines the sugar type and its isomers. The representations of common monosaccharides and linkages are shown above (as illustrated in the "Essentials of Glycobiology" reference below).

How many labeled/unlabeled glycan pairs do you offer?

Our current inventory is 11 pairs, but please inquire if a corresponding analogue of an existing standard is required or a certain new pair is of interest.

How many N-glycan standards do you offer?

We currently offer 32 N-linked glycans. Please inquire for the availability of other N-glycans.

What is the recommended reconstitution condition for the glycans?

The glycans can be solubilized in water or a buffered solution to the desired concentration. General precautions should be adhered to (i.e., mix well and spin before opening, aliquot if needed, store at ≤-20°C if in solution, avoid repeated freeze-thaws).

Can alternate glycans be made available upon request?

Yes, provided they can be synthesized through enzymatic and/or chemical means. Please submit your request to your local sales representative/distributor or complete the on-line custom synthesis form. Required is the structure(s), desired quantities, and labeling pattern(s) (if applicable). We will contact you shortly thereafter regarding its feasibility.

References

Taniguchi, N.; Kizuka, Y. 2015. Glycans and cancer: role of N-glycans in cancer biomarker, progression and metastasis, and therapeutics. Adv Cancer Res, 126, 11-51. PMID: 25727145

Schedin-Weiss, S.; Winblad, B.; Tjernberg, L.O. 2014. The role of protein glycosylation in Alzheimer disease. FEBS J, 281(10), 46-62. PMID: 24279329

Varki, A; Cummings, R.D.; Esko, J.D.; et al. 2009. Essentials of Glycobiology, 2nd edition. ISBN-13:9780879697709. www.ncbi.nlm.nih.gov/books/NBK1908/

Andrew Percy, PhD

Senior Applications Chemist – Mass Spectrometry

Dr. Andrew Percy is the Senior Applications Chemist for Mass Spectrometry and the MS ‘Omics Product Manager at CIL. His responsibilities minimally involve providing technical support, overseeing product development, identifying new product market opportunities, assisting in the analysis of product-related applications, and writing/reviewing marketing literature.

Kevin Millis, PhD

Senior Scientist, Application Development Manager

Kevin Millis, PhD, is the Senior Scientist and Market Development Manager for all NMR and mass spectrometry product lines. Kevin is responsible for Technical Services both internally and externally for all CIL customers as well as being responsible for the application and market development for the CIL products.