Enzymatic Labeling
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- 18O Labeling
The incorporation of two 18O atoms into each C-terminus of peptides derived from proteolytic digestion of biological samples has emerged as one of the leading global labeling strategies used in comparative quantitative proteomics. The success of the technique is due in part to the relative low cost of 18O water, the resulting +4 Dalton mass increase in molecular weight for the “heavy” peptide, and co-elution of 18O/16O peptide pairs from reverse-phase HPLC.
References
Farthing D.E.; Buxbaum, N.P.; Lucas, P.J.; et al. 2017. Comparing DNA enrichment of proliferating cells following administration of different stable isotopes of heavy water. Sci Rep, 7(1), 4043. PMID: 28642474
Liu, M.; Zhang, Z.; Cheetham, J.; et al. 2017. Discovery and characterization of a photo-oxidative histidine-histidine cross-link in IgG1 antibody utilizing ¹⁸O-labeling and mass spectrometry. Anal Chem, 86(10), 4940-4948. PMID: 24738698
Liu, M.; Zhang, Z., Zang, T.; et al. 2013. Discovery of undefined protein cross-linking chemistry: a comprehensive methodology utilizing 18O-labeling and mass spectrometry. Anal Chem, 85(12), 5900-5908. PMID: 23634697
Auclair, J.R.; Somasundaran, M.; Green, K.M., et al. 2012. Mass spectrometry tools for analysis of intermolecular interactions. Methods Mol Biol, 896, 387-398. PMID: 22821539
Fenselau, C.; Yao, X. 2009. 18O2-labeling in quantitative proteomic strategies: a status report. J Proteome Res, 8(5), 2140-2143. PMID: 19338309

Andrew Percy, PhD
Senior Applications Chemist – Mass Spectrometry
Dr. Andrew Percy is the Senior Applications Chemist for Mass Spectrometry and the MS ‘Omics Product Manager at CIL. His responsibilities minimally involve providing technical support, overseeing product development, identifying new product market opportunities, assisting in the analysis of product-related applications, and writing/reviewing marketing literature.
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Kevin Millis, PhD
Senior Scientist, Application Development Manager
Kevin Millis, PhD, is the Senior Scientist and Market Development Manager for all NMR and mass spectrometry product lines. Kevin is responsible for Technical Services both internally and externally for all CIL customers as well as being responsible for the application and market development for the CIL products.
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