In situ Protein Expression
Share
- Cell-Free Production of Stable Isotope-Labeled Proteins
- In vitro Protein Synthesis of Perdeuterated Proteins for NMR Studies (Application Note 19)
- CFS Products
- Wheat Germ Systems for Cell-Free Protein Expression
Cell-Free Protein Expression
CIL offers a wide variety of products for cell-free protein expression. Cell-free protein expression methods offer several advantages over expression using E. coli or other in vivo expression systems. These advantages include increased speed, ability to express toxic proteins, ease of amino acid type selective labeling, and an open system that allows cofactors, chaperones, redox molecules, and detergents to be easily be added to the expression system. Cell-free methods also allow co-expression of multiple proteins and are amenable to automation.
CIL is proud to distribute a wide range of products from CellFree Sciences (CFS). CIL also offers algal-derived amino acid mixes and conveniently packaged sizes of individual crystalline amino acids.
Resources
Introduction to Wheat Germ Cell-Free Protein Expression
Cell-Free Synthesis of Labeled Proteins
FLEXIQuant PLUS Expression Kit
ProteoLiposome Expression Kits
Disulfide Bond Expression Kits
Selected References in Wheat Germ Cell-Free Protein Expression
Reference
Fogeron, M.-L.; Lecoq, L.; Cole, L.; et al. 2021. Easy synthesis of complex bimolecular assemblies: wheat germ cell-free protein expression in structural biology. Front Mol Biosci, 8, 639587. PMID: 33842544
Kevin Millis, PhD
Senior Scientist, Application Development Manager
Kevin Millis, PhD, is the Senior Scientist and Market Development Manager for all NMR and mass spectrometry product lines. Kevin is responsible for Technical Services both internally and externally for all CIL customers as well as being responsible for the application and market development for the CIL products.
More
