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Biomolecular NMR

In situ Protein Expression

Cell-Free Production of Stable Isotope-Labeled Proteins


  • Cell-Free Production of Stable Isotope-Labeled Proteins
  • In vitro Protein Synthesis of Perdeuterated Proteins for NMR Studies (Application Note 19)
  • CFS Products
  • Wheat Germ Systems for Cell-Free Protein Expression

 

Cell-Free Protein Expression

CIL offers a wide variety of products for cell-free protein expression. Cell-free protein expression methods offer several advantages over expression using E. coli or other in vivo expression systems. These advantages include increased speed, ability to express toxic proteins, ease of amino acid type selective labeling, and an open system that allows cofactors, chaperones, redox molecules, and detergents to be easily be added to the expression system. Cell-free methods also allow co-expression of multiple proteins and are amenable to automation.

CIL is proud to distribute a wide range of products from CellFree Sciences (CFS). CIL also offers algal-derived amino acid mixes and conveniently packaged sizes of individual crystalline amino acids.
 

Resources

  CFS Products

  Introduction to Wheat Germ Cell-Free Protein Expression

  Cell-Free Synthesis of Labeled Proteins

  WEPRO7240 Core Kits

  WEPRO8240 Core Kits

  FLEXIQuant PLUS Expression Kit

  Premium ONE Expression Kits

  Premium PLUS Expression Kits

  Protein Research Kits

  pEU Vector Set

  ProteoLiposome Expression Kits

  Disulfide Bond Expression Kits

  Selected References in Wheat Germ Cell-Free Protein Expression

  Amino Acids for NMR


Reference

Fogeron, M.-L.; Lecoq, L.; Cole, L.; et al. 2021. Easy synthesis of complex bimolecular assemblies: wheat germ cell-free protein expression in structural biology. Front Mol Biosci, 8, 639587. PMID: 33842544

 

Kevin Millis, PhD

Kevin Millis, PhD

Senior Scientist, Application Development Manager

Kevin Millis, PhD, is the Senior Scientist and Market Development Manager for all NMR and mass spectrometry product lines. Kevin is responsible for Technical Services both internally and externally for all CIL customers as well as being responsible for the application and market development for the CIL products.

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