Organic Acid Mix

Metabolomics Organic Acid Mix

Metabolomics Organic Acid Mix
Organic Acid Quantitation in Mouse Muscle by Ion Chromatography-Mass Spectrometry with Isotopically Labeled Standards (Application Note 47)

Organic acids (OAs) play essential roles in energy metabolism pathways (e.g., glycolysis, tricarboxylic acid cycle), with the short-chained OAs emerging as important regulators of host immune response and transcriptional regulation. OAs are byproducts of amino acids and are intermediates in various biochemical pathways. To aid MS-based metabolomic research endeavors, CIL has formulated an isotope-enriched organic acid mix. This single-vial mixture comprises 33 organic acids (MSK-OA-1) and is also available as an unlabeled mix (MSK-OA-US-1).

Note: Mixes of the organic acid subclasses may be available; please inquire.

Resources

Metabolomics Organic Acid Mix

S ets, Mixes, and Kits for MS 'Omics and MS/MS Screening

Stable Isotope Standards for Mass Spectrometry

Organic Acid Quantitation in Mouse Muscle by Ion Chromatography-Mass Spectrometry (Application Note 47)

Reference

DeArmond, P.D.; Bunch, D.R. 2022. Quantitation of non-derivatized free amino acids for detecting inborn errors of metabolism by incorporating mixed-mode chromatography with tandem mass spectrometry. J Mass Spectrom Adv Clin Lab, 25, 1-11. PMID: 35637738

Frequently Asked Questions

What is an appropriate solvent for reconstituting the dried-down OA mixes?

Concentrated stock solutions of the labeled and unlabeled OA mixes can be prepared in aqueous solvents such as water or 0.1% formic acid (FA). Dilutions of working stocks for control and experimental sample use can then be prepared as necessary.

How many TCA cycle metabolites are present in the OA mixes?

There are six TCA-related metabolites in the mix (MSK-OA). These metabolites include two keto acids (α-ketoglutarate and pyruvate), two diacids (succinate and fumarate), one hydroxy acid (malate), and one other acid (citrate). In addition to these intermediates, there are two OAs (DL-2-hydroxyglutarate and lactate) that are off-shoots to the TCA cycle, but could also be considered related.

How have the mixes been measured?

All metabolites in the master mixes have been measured by HILIC-MS on an Orbitrap ID-X mass spectrometer (negative ESI). Concentrated stocks of the metabolite subsets have effectively been measured by derivatization-based GC-MS.

If alternate organic acids are needed, can additional standards be provided?

Yes, we offer a breadth of individual stable isotope labeled organic acids beyond what is included in the mix. Please click here for the listing. In the event that your usage is routine, we can evaluate a custom mix or add-on vial to meet your needs. Refer to the Custom Mix Request form to complete your request for quotation or contact your local sales representative.

Krista Backiel

Marketing Manager and Metabolomics Manager

Krista Backiel is responsible for managing and promoting products that are utilized in metabolomics and clinical/diagnostic MS. She spends a lot of her time developing new products to assist customers in their diverse research efforts.

Andrew Percy, PhD

Senior Applications Chemist – Mass Spectrometry

Dr. Andrew Percy is the Senior Applications Chemist for Mass Spectrometry and the MS ‘Omics Product Manager at CIL. His responsibilities minimally involve providing technical support, overseeing product development, identifying new product market opportunities, assisting in the analysis of product-related applications, and writing/reviewing marketing literature.