Dimethyl Labeling

The dimethyl labeling technique uses a reagent mixture (i.e., cyanoborohydride and formaldehyde in their unlabeled and stable isotope-labeled forms) to tag primary amines (i.e., the N-terminus and the ε-amino group of lysine) in proteins or peptides. This is a fast, straightforward, and inexpensive approach to conduct 2- or 3-plex quantitative proteomic analyses of a variety of sample types (e.g., lysate, tissue). To facilitate reductive amination reactions in quantitative proteome profiling (example provided in App Note 38), CIL is pleased to offer a variety of chemical tagging reagents.

➤ Stable Isotope Standards for Mass Spectrometry

Stable Isotope Dimethyl Labeling

Frequently Asked Question

At what stage of a bottom-up proteomics experiment are the dimethyl labeling reagents inserted? The reductive methylation reagents (i.e., formaldehyde and cyanoborohydride) are typically added to peptides post-digestion.

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