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The Isotope Gateway


The primary purpose of presenting a scientific poster at a conference or workshop is to allow and encourage direct discourse between peers so that existing research goals can better be met and perhaps new ones developed. Certainly, preparing and presenting a scientific poster is an important step of any graduate or post-doctoral student's career. The preparation of a successful poster requires not only scientific knowledge, but creativity and artistry to best convey the research story.

CIL recognizes the importance of scientific posters as a tool to disperse scientific knowledge and appreciates the effort needed to create them. Unfortunately, posters at a meeting are typically available for viewing for only a few days at most. This is one reason why CIL has established the Isotope Gateway.

Here is where CIL will give access to a collection of scientific posters and a bibliography of published scientific papers, all of which describe work utilizing stable isotope-labeled compounds. 

  • Scientific Posters
  • Reference Library




Absolute Quantification of Histone Deacetylase Isoforms in Alzheimer’s Disease Brain

Kyle W. Anderson and Illarion V. Turko

Incorporation Rate of Lysine-13C6 in Non-Generational Metabolic Labeling in Mice

Kevin Millis, Michael Ford, Richard Jones, Tasha Agreste, John Rod

In vivo Quantitative Proteomics of Somatosensory Cortical Synapses Shows Which Protein Levels Are Modulated by Sensory Deprivation

Jeffrey N. Savas, Margaret T. Butko, Beth Friedman, Claire Delahunty, Ford Ebner, Roger Y. Tsien, John R. Yates III


Protein Expression

Top 10 Tips for Producing 13C, 15N Protein in Abundance

Deborah A. Berthold, Victoria J. Jeisy, Terry L. Sasser, John J. Shea, Heather L. Freicks, Gautan Shan, Chad M. Rienstra

Stable Istotope Labeled Cell Growth Media for Insect, Yeast and Mammalian Cells

Lynne S. Batchelder, PhD, Senior Chemist
Cambridge Isotope Laboratories, Inc.

The Production and Use of a NIST-Traceable Standard Solution for Quantitative NMR Applications

Kris Dziewiszek, Kevin Millis, Joe Ray, Tim Eckersley
Cambridge Isotope Laboratories, Inc. and Baxter Healthcare Corporation

Hyperpolarized [1-13C] Pyruvate for Noninvasive Examination of Placental Metabolism and Nutrient Transport: A Feasibility Study in Pregnant Guinea Pigs

Lanette J. Friesen-Waldner, PhD, et al.
Department of Medical Biophysics, University of Western Ontario, London, Ontario, Canada


Metabolic Research 


Profiling of Polar Organic Acids in Mouse Muscle Using Ion Chromatography/Mass Spectrometry 

Christopher Petucci1,2, Andrew V. Zelenin1,2, Jeffrey A. Culver1,2, Meghan Gabriel1, Ken Kirkbride3, Terri T. Christison3, and Stephen J. Gardell1,2

1. Metabolomics Core Facility, Sanford Burnham Prebys Medical Discovery Institute, 6400 Sanger Road, Orlando, FL, USA
2. Southeast Center for Integrated Metabolomics (SECIM), Clinical and Translational Science Institute, 2004 Mowry Road, Gainesville, FL, USA
3. Thermo Fisher Scientific, 1214 Oakmead Parkway, Sunnyvale, CA, USA


Pathway Targeted Metabolomic Analysis in Oral Cancer Cells Using High Performance Ion Chromatography Coupling to A New HR/AM Orbitrap Mass Spectrometry

Junhua Wang1, Terri Christison1, Krista Backiel2, Grace Ji3, Shen Hu3, Linda Lopez1, Yingying Huang1

1. Thermo Fisher Scientific Inc., San Jose, CA
2. Cambridge Isotope Laboratories, Inc., MA
3. School of Dentistry and Jonsson Comprehensive Cancer Center, UCLA, Los Angeles, CA
Daniel Krug1,2, Carsten Volz1, Aiko Barsch3, Chris Beecher4, Felice de Jong4, Rolf Müller1,2
1. Helmholtz Institute for Pharmaceutical Research, Saarland (HIPS), Saarbrücken and Helmholtz Center for Infection Research (HZI), Braunschweig, Germany
2. Saarland University, Saarbrücken, Germany
3. Bruker Daltonik GmbH, Bremen, Germany
4. IROA Technologies, Ann Arbor, Michigan, USA

Bioprocess of a Yeast Fermentation Using the IROA Protocol

Chris Beecher and Felice de Jong
IROA Technologies LLC, Ann Arbor, MI, USA


Betaine Is as Effective as Folate at Re-synthesizing Methionine for Protein Synthesis During Moderate Methionine Deficiency in Piglets 

Laura E. McBreairty, Jason L. Robinson, Scott Harding, Edward Randell, Janet A. Brunton, Robert F. Bertolo 

The Impact of Dietary Methyl Donors on Whole-Body Methionine Partitioning in the Neonatal Piglet

Jason L. Robinson1, Laura E. McBreairty1, Scott V. Harding2, Janet A. Brunton1, Robert F. Bertolo1
1. Department of Biochemistry, Memorial University of Newfoundland, St. John’s, NL, Canada
2. Division of Nutrition Sciences, School of Medicine, Kings College London, London, UK

Isotopically Labeled Argininosuccinic Acid: A New Reference Standard for ASA

Adrienne Manning,1 William Wood,2 and Susan Henke2
1. Connecticut Department of Public Health Laboratory, Rocky Hill, CT
2. Cambridge Isotope Laboratories, Inc., Andover, MA

Breath Tests

Companion Diagnostic (CDx) for Personalizing Psychiatric Medications: Clinical Unmet Need

Anil Modak, PhD
Cambridge Isotope Laboratories, Inc. 3 Highwood Drive, Tewksbury, MA 01876 USA


Proteomics Mass Spectrometry Biomolecular NMR
Metabolic Research NMR Solvents and Consumables Standards for Environmental Analysis



Mass Spectrometry


Amplatz, B.; et al. 2017. Bile acid preparation and comprehensive analysis by high performance liquid chromatography-high-resolution mass spectrometry. Clin Chim Act, 464, 85-92. PMID: 27838249

Wegner, K.; et al. 2017. Rapid analysis of bile acids in different biological matrices using LC-ESI-MS/MS for the investigation of bile acid transformation by mammalian gut bacteria. Anal Bioanal Chem, 409(5), 1231-1245. PMID: 27822648

Sarafian, M.H.; et al. 2015. Bile acid profiling and quantification in biofluids using ultra-performance liquid chromatography tandem mass spectrometry. Anal Chem, 87(19), 9662-9670. PMID: 26327313

Han, J.; et al. 2015. Metabolic profiling of bile acids in human and mouse blood by LC-MS/MS in combination with phosopholipid-depletion solid-phase extraction. Anal Chem, 87(2), 1127-1136. PMID: 25496250

Gertsman, I.; et al. 2014. Validation of a dual LC-HRMS platform for clinical metabolic diagnosis in serum, bridging quantitative analysis and untargeted metabolomics. Metabolomics, 10(2), 312-323. PMID: 25411574

Suzuki, Y.; et al. 2013. Simple and rapid quantitation of 21 bile acids in rat serum and liver by UPLC-MS-MS: effect of high fat diet on glycine conjugates of rat bile acids. Nagoya J Med Sci, 75(1-2), 57-71. PMID: 23544269

Schrire, B.D.; et al. 2002. Indigofera mauritanica internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene, complete sequence; and internal transcribed spacer 2, partial sequence. Adv Legume Syst, 10. NCBI: AF521794.1



Biomolecular NMR 


Harbers, M. 2014. Wheat germ systems for cell-free protein expression. FEBS Lett, 588(17), 2762-2773. PMID: 24931374

Sinnige, T.; Daniels, M.; Baldus, M.; et al. 2014. Proton Clouds to Measure Long-Range Contacts between Nonexchangeable Side Chain Protons in Solid-State NMR. J Am Chem Soc, 136(12), 4452-4455. PMID: 24467345

Weingarth, M.; Baldus., M. 2013.  Solid-State NMR-Based Approaches for Supramolecular Structure Elucidation. Acc Chem Res, 46(9), 2037-2046. PMID: 23586937

Loquet, A.; Habenstein, B.; Lange, A. 2013. Structural Investigations of Molecular Machines by Solid-State NMR. Acc Chem Res, 46(9), 2013-2079. PMID: 23496894

Verardi, R.; Traaseth, N.J.; Masterson, L. R.; et al. 2012. Isotope Labeling for Solution and Solid-State NMR Spectroscopy of Membrane Proteins. Adv Ex Med Bio, 992, 35-62. PMID: 23076578

Huber, M.; Hiller, S.; Schanda, P.; et al. 2012. A Proton-Detected 4D Solid-State NMR Experiment for Protein Structure Determination. Chem Phys Chem, 12(5), 915-918. PMID: 21442705

Park, S.H.; Das, B.B.; Casagrande. F.; et al. 2012. Structure of the chemokine receptor CXCR1 in phospholipid bilayers. Nature, 491(7426), 779-783. PMID: 23086146

Takeuchi, K.; Frueh, D.P.; Sun, Z.Y.; et al. 2010. HNCA-TOCSY-CANH experiments with alternate 13C-12C labeling: a set of 3D experiment with unique supra-sequential information for mainchain resonance assignment. J Biomol NMR, 49(1), 17-26. PMID: 21110064

Thakur, C.S.; Sama, J.N.; Jackson, M.E.; et al. 2010. Selective 13C labeling of nucleotides for large RNA NMR spectroscopy using an E. coli strain disabled in the TCA cycle. J Biomol NMR, 48(4), 179-192. PMID: 21057854

Wagner, G.; Lu, K.; Miyazaki, Y.; et al. 2010. A topical issue: production and labeling of biological macromolecules for NMR investigations J Biomol NMR, 46(1), 113-125. PMID: 19924385

Krishnarjuna, B.; Jaiupuria, G.; Thakur, A.D.; et al. 2010. Amino acid selective unlabeling for sequence specific resonance assignments in proteins. J Biomol NMR, 49(1), 38-51. PMID: 21153044

Zhu, J.; Ye, E.; Terskikh, V.; et al. 2010. Solid-state 17O NMR spectroscopy of large protein-ligand complexes. Angew Chem Int Ed, 49(45), 8399-8402. PMID: 20672261

Takahashi, H.; Shimada, I. 2010. Production of isotopically labeled heterologous proteins in non-E. coli prokaryotic and eukaryotic cells. J Biomol NMR, 46(1), 3-10. PMID: 19787297

Filipp, F.V.; Sinha, N.; Jairam, L.; et al. 2009. Labeling strategies for 13C-detected aligned-sample solid-state NMR of proteins. J Magn Reson, 201(2), 121-130. PMID: 19781966

Ayala, I.; Sounier, R.; Use, N.; et al. 2009. An efficient protocol for the complete incorporation of methyl-protonated alanine in perdeuterated protein. J Biomol NMR, 43(2), 111-119. PMID: 19115043

Wylie, B.J. 2009. Protein Structure Refinement Using 13Cα Chemical Shift Tensors. J Am Chem Soc, 131(3), 985-992. PMID: 19123862

Tong, K.I.; Yamamoto, M.; Tanaka, T. 2008. A simple method for amino acid selective isotope labeling of recombinant proteins in E. coli. J Biomol NMR, 42(1), 59-67. PMID: 18762866

Hiller, S.; Garces, R.G.; Malia, T.J.; et al. 2008. Solution structure of the integral human membrane protein VDAC-1 in detergent micelles. Science, 321(5893), 1206-1210. PMID: 18755977

Zhou, Y.; Cierpicki, T.; Jimenez, R.H.; et al. 2008. NMR solution structure of the integral membrane enzyme DsbB: functional insights into DsbB-catalyzed disulfide bond formation. Mol Cell, 31(6), 896-908. PMID: 18922471

Takeuchi, K.; Ng, E.; Malia, T.J.; et al. 2007. 1-13C amino acid selective labeling in a 2H15N background for NMR studies of large proteins. J Biomol NMR, 38(1), 89-98. PMID: 17390105

Isaacson, R.L.; Simpson, P.J.; Liu, M.; et al. 2007. A new labeling method for methyl transverse relaxation-optimized spectroscopy NMR spectra of alanine residues. J Am Chem Soc, 129(50), 15428-15429. PMID: 18041839

Kainosho, M.; Torizawa, T.; Iwashita, Y.; et al. 2006. Optimal isotope labeling for NMR protein structure determinations. Nature, 440(7080), 52-57. PMID: 16511487

Frueh, D.P.; Vosburg, D.A.; Walsh, C.T.; et al. 2006. Non-uniformly sampled double-TROSY hNcaNH experiments for NMR sequential assignments of large proteins. J Am Chem Soc, 128(17), 5757-5763. PMID:16637644

Chen, C.Y.; Cheng, C.H.; Chen, Y.C.; et al. 2006. Preparation of amino-acid-type selective isotope labeling of protein expressed in Pichia pastoris. Proteins, 62(1), 279-287. PMID: 16283643

Davis, J.H.; Tonelli, M.; Scott, L.G.; et al. 2005. RNA Helical packing in solution: NMR structure of a 30 kDa GAAA tetraloop-receptor complex. J Mol Biol, 351(2), 371-382. PMID: 16002091

Tugarinov, V.; Kay, L.E. 2005. Methyl groups as probes of structure and dynamics in NMR studies of high-molecular-weight proteins. Chembiochem, 6(9), 1567-1577. PMID: 16075427

Tyler, R.C.; Sreenath, H.K.; Singh, S.; et al. 2005. Auto-induction medium for the production of [U-15N]- and [U-13C, U-15N]-labeled proteins for NMR screening and structure determination. Protein Expr Purif, 40(2), 268-278. PMID: 15766868

Torizawa, T.; Shimizu, M.; Taoka, M.; et al. 2004. Efficient production of isotopically labeled proteins by cell-free synthesis: A practical protocol. J Biomol NMR, 30(3), 311-325. PMID: 15754057

Pickford, A.R.; O‘Leary, J.M. 2004. Isotopic labeling of recombinant proteins from the methylotrophic yeast Pichia pastoris. Methods Mol Biol, 278, 17-33. PMID: 15317988

Rodriguez-Mias, R.; Pellecchia, M. 2003. Use of Selective Trp Side Chain Labeling to Characterize Protein-Protein and Protein-Ligand Interactions by NMR Spectroscopy. J Am Chem Soc, 125(10), 2892-2893. PMID: 12617653

Castellani, F.; van Rossum, B.; Diehl, A.; et al. 2002. Structure of a protein determined by solid-state magic-angle-spinning NMR spectroscopy. Nature, 420(6911), 98-102. PMID: 12422222

Hwang, P.M.; Choy, W.Y.; Lo, E.I.; et al. 2002. Solution structure and dynamics of the outer membrane enzyme PagP by NMR. Proc Natl Acad Sci USA, 99(21), 13560-13565. PMID: 12357033

Rodriguez, E.; Krishna, N.R. 2001. An economical method for 15N / 13C isotopic labeling of proteins expressed in Pichia pastoris. J Biochem, 130(1), 19-22. PMID: 11432775

Cowburn, D. and Muir, T.W. 2001. Segmental isotopic labeling for structural biological applications of NMR. Methods Mol Biol, 278, 47–56. PMID: 15317990

Atreya, H.S.; Chary, K.V.R. 2001. Selective unlabeling of amino acids in fractionally 13C-labeled proteins: An approach for stereospecific NMR assignments of CH3 groups in Val and Leu residues. J Biomol NMR, 19(3), 267-272. PMID: 11330814

Morgan, W.D.; Kragt, A.; Feeney, J. 2000. Expression of deuterium-isotope-labeled protein in the yeast Pichia pastoris for NMR studies. J Biomol NMR, 17(4), 337-347. PMID: 11014598

Goto, N.K.; Gardner, K.H.; Mueller, G.A.; et al. 1999. A robust and cost-effective method for the production of Val, Leu, Ile (δ1) methyl-protonated 15N-, 13C-, 2H-labeled proteins. J Biomol NMR, 13(4), 369-374. PMID: 10383198

Wood, M.J.; Komives, E.A. 1999. Production of large quantities of isotopically labeled protein in Pichia pastoris by fermentation. J Biomol NMR, 13(2), 149-159. PMID: 10070756

Massou, S.; Puech, V.; Talmont, F.; et al. 1999. Heterologous expression of a deuterated membrane-integrated receptor and partial deuteration in methylotrophic yeasts. J Biomol NMR, 14(3), 231-239. PMID: 10481275

Hong, M. 1999. Determination of multiple phi-torsion angles in proteins by selective and extensive 13C labeling and two-dimensional solid-state NMR. J Magn Reson, 139(2), 389-401. PMID: 10423377

Gardner, K.H.; Kay, L.E. 1997. Production and Incorporation of 15N, 13C, 2H (1H δ1 methyl) Isoleucine into Proteins for Multidimensional NMR Studies. J Am Chem Soc, 119(32), 7599-7600. JACS DOI: 10.1021/ja9706514

Batey, R.T. 1996. Improved large-scale culture of Methylophilus methylotrophus for 13C / 15N labeling and random fractional deuteration of ribonucleotides. Nucleic Acids Res, 24(23), 4836-4837. PMID: 8972874

Vuister, G.W.; Kim, S.J.; Wu, C.; et al. 1994. 2D and 3D NMR study of phenylalanine residues in proteins by reverse isotopic labeling. J Am Chem Soc, 116(20), 9206-9210 JACS DOI:10.1021/ja00099a041

Ikura, M.; Kay, L.E.; Bax, A. 1990. A novel approach for sequential assignment of 1H, 13C, and 15N spectra of larger proteins: heteronuclear triple-resonance three-dimensional NMR spectroscopy. Application to Calmodulin. Biochemistry, 29(19), 4659-4667. PMID: 2372549


Metabolic Research


Geary, B.; Magee, K.; Cash, P.; et al. 2016. Determining synthesis rates of individual proteins in zebrafish (Danio rerio) with low levels of a stable isotope labelled amino acid. Proteomics, 16(9), 1398-1406. PMID: 26929125

Sellers, K.; Fox, M.; Bousamra, M.; et al. 2015. Pyruvate carboxylase is critical for non-small-cell lung cancer proliferation. J Clin Invest, 125(2), 687-698. PMID: 25607840

Buescher, J.; Antoniewicz, M.; Boros, L.; et al. 2015. A roadmap for interpreting 13C metabolite labeling patterns from cells. Curr Opin Biotechnol 34, 189-201. PMID: 25731751

Mayers, J.R., et al., 2014. Elevation of circulating branched-chain amino acids is an early event in human pancreatic adenocarcinoma development. Nat Med, 20(10), 1193-1208. PMID: 25261994

Lewis, C., et al. 2014. Tracing Compartmentalized NADPH Metabolism in the Cytosol and Mitochondria of Mammalian Cells. Mol Cell, 55(2), 253-263. PMID: 24882210

Fan, J.; Ye, J.; Kamphorst, J.; et al. 2014. Quantitative flux analysis reveals folate-dependent NADPH production. Nature, 510(7504), 298-302. PMID: 24805240

Marini, J.C., et al. 2012. Arginine and Ornithine Are the Main Precursors for Citrulline Synthesis in Mice. J Nutrition, 142(3), 572-580. PMID: 22323761



Shwaiger, M.; Rampler, E.; Hermann, G.; et al. 2017. Anion-exchange chromatography coupled to high resolution mass spectrometry: a powerful tool for merging targeted and non-targeted metabolomics. Anal Chem, 89(14), 7667-7674. PMID: 28581703

Percy, A.J.; Byrns, S.; Pennington, S.R.; et al. 2016. Clinical translation of MS- based, quantitative plasma proteomics: status, challenges. Expert Rev Proteomics, 13(7), 673-684. PMID: 27341553 

Bao, X.R.; Ong, S.E.; Goldberger, O.; et al. 2016. Mitochondrial dysfunction remodels one-carbon metabolism in human cells. Elife, 16(5), e10575. PMID: 27307216 

McConnell, B.O.; Antoniewicz, M.R. 2016. Measuring the Composition and Stable-Isotope Labeling of Algal Biomass Carbohydrates via Gas Chromatography/Mass Spectrometry. Anal Chem, 88(9), 4624-4628. PMID: 27042946

Baughman, J.M.; Rose, C.M.; Koluman, G.; et al. 2016. NeuCode Proteomics Reveals Bap1 Regulation of Metabolism. Cell Rep,16(2), 583-595. PMID: 27373151

Geary, B.; Magee, K.; Cash, P.; et al. 2016. Determining synthesis rates of individual proteins in zebrafish (Danio rerio) with low levels of a stable isotope labelled amino acid. Proteomics, 16(9), 1398-1406. PMID: 26929125

Hecht, E.S.; McCord, J.P.; Muddiman, D.C. 2016. A Quantitative Glycomics and Proteomics Combined Purification Strategy. J Vis Exp, 8(109). PMID: 27023253

Baughman, JM; Rose, CM; Koluman, G. 2016. NeuCode Proteomics Revearls Bap1 Regulation of Metabolism. Cell Rep, 16(2), 583-595PMID: 27373151 

Singh, S.A.; Andraski, A.B.; Pieper, B.; et al. 2016. Multiple apolipoprotein kinetics measured in human HDL by high-resolution/accurate mass parallel reaction monitoring. J Lipid Res, 57(4), 714-728. PMID: 26862155

Henderson, C.M.; Vaisar, T.; Hoofnagle, A.N. 2016. Isolating and Quantifying Plasma HDL Proteins by Sequential Density Gradient Ultracentrifugation and Targeted Proteomics. Methods Mol Biol, 1410, 105-20. PMID: 26867741

Zhang, A.; Uaesoontrachoon, K.; Shaughnessy, C.; et al. 2015









To Submit a Poster 

1. Please send a PDF of your poster to The poster must describe the utilization of stable isotope-labeled compounds in a research application. 
2. You will be sent a confirmation email and a permission waiver to sign if your poster has been approved to be included in the poster archive.

To Submit a Literature Reference 

1. Please e-mail your literature reference to Include an open-access PDF version of the paper. The submitter of the reference must be listed as an author, and the paper must describe the use of a stable isotope. 
2. You will be sent a confirmation email if the literature reference has been accepted into the bibliography.

Be Rewarded!

If your poster or literature reference submission has been reviewed and approved and either mentions CIL stable isotopes and/or shows the CIL logo you will receive a CIL fleece jacket* as a thank you from us! Stay tuned for future incentives!


The Isotope Gateway | Cambridge Isotope Laboratories
Poster push, stable isotopes, scientific poster, referenced, bibliographies,
Poster push, stable isotopes, scientific poster, referenced, bibliographies, stable isotopes