PeptiQuant™

Inter-laboratory evaluation of instrument platforms and experimental workflows for quantitative accuracy and reproducibility assessment

Andrew J. Percy, Jessica Tamura-Wells, et al.

University of Victoria - Genome British Columbia Proteomics Centre, Victoria, British Columbia, Canada


  • Inter-laboratory evaluation of instrument platforms and experimental workflows for quantitative accuracy and reproducibility assessment
  • Advances in multiplexed MRM-based protein biomarker quantitation toward clinical utility
  • A standardized kit for automated quantitative biomarker assessment of candidate protein biomarkers in human plasma
  • Qualis-SIS: Automated Standard Curve Generation and Quality Assessment for Multiplexed Targeted Quantitative Proteomic Experiments with Labeled Standards
Researchers in academia and life science industries continue to adopt a targeted, bottom-up MS-based proteomic workflow for biomarker discovery and validation. Biomarker validation requires absolute quantification of surrogate peptides in the sample matrix, a requirement that is best achieved using stable isotope-labeled standard (SIS) peptides. Although the use of SIS has increased the reliability of LC-MRM/MS-based assays, optimal results require properly functioning equipment and a workflow with minimal human error and bias. 
 
To help researchers establish a stable LC-MRM/MS platform for bottom-up quantitative proteomics, Cambridge Isotope Laboratories, Inc. is pleased to offer PeptiQuant™ kits from MRM Proteomics Inc. These kits include the PeptiQuant™ Monthly LC-MS Platform Performance Kit, the PeptiQuant™ Daily LC-MS Platform Performance Kit, and the PeptiQuant™ Workflow Performance Kit. These innovative products are used to assess and track LC-MS performance of the LC-MS platform in a proteomic workflow in order to highlight any possible issues affecting quantitation. 
 
CIL is also pleased to offer a number of PeptiQuant™ Biomarker Assessment Kits (BAKs; e.g., BAK-76), to help researchers monitor panels of candidate protein biomarkers in human/mouse plasma. This BAK-76, for example,contains the reagents and software tools necessary to measure up to 76 proteins of biological importance in human plasma samples.
 

 

Resources

 

For further information on these products, please visit MRM Proteomics at  http://www.mrmproteomics.com

 

References

 

Percy, A.J.; Michaud,SA; Jardim, A. 2016. Multiplexed MRM-based assays for the quantitation of proteins in mouse plasma and heart tissue. Proteomicsdoi: 10.1002/pmic.201600097PMID: 27688154

Hirtz,C.; Vialaret, J.; Nowak, N. 2016. Absolute quantification of 35 plasma biomarkers in human saliva using targeted MS. Bioanalysis. 8(1):43-53. PMID: 26647646

Percy, A.J.; Mohammed, Y.; Yang, J. 2015. A Standard kit for automated quantitative assessment of candidate protein biomakers in human plasma. Bioanalysis, 7(23):2991-3004. PMID: 26626236

Mohammed, Y.; Percy, A.J.; Chambers, A.G. 2015. Qualis-SIS: automated standard curve generation and quality assessment for multiplexed targeted quantitative proteomic experiments with labeled standards.J Proteome Res., 14(2):1137-46. PMID: 25546269

Percy, A.J.; Simon, R.; Chambers, A.G.; Borchers, C.H. 2014. Enhanced sensitivity and mulitplexing with 2D LC/MRM-MS and labeled standards for deeper and more comprehensive protein quantitation. J Proteomics, 25;106:113-24 PMID: 24769237

Percy, A.J.; Chambers, A.G.; Yang, J. 2013. Method and platform standardization in MRM-based quantitative plasma proteomics. J Proteomics, 16;95:66-76. PMID: 23933160

Percy, A.J.; Chambers, A.G.; Smith, D.S. 2013. Standardized protocols for quality control of MRM-based plasma proteomic workflows. J Proteome Res., 12(1):222-33. PMID: 23245390

Kevin Millis, PhD

Kevin Millis, PhD

Senior Scientist, Application Development Manager

Kevin Millis, PhD, is the Senior Scientist and Market Development Manager for all NMR and Mass Spectrometry product lines. Kevin is responsible for Technical Services both internally and externally for all CIL customers as well as being responsible for the application and market development for the CIL products.

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Andrew Percy, PhD

Andrew Percy, PhD

Senior Applications Chemist - Mass Spectrometry

Dr. Andrew Percy is the Senior Applications Chemist for Mass Spectrometry. His responsibilities minimally involve overseeing product development, identifying new product market opportunities, assisting in the analysis of products for MS ‘omics applications, and providing technical support to customers.

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Tasha Agreste

Tasha Agreste

Deuterated Reagents & Intermediates Product Manager, China Sales - Research Products

Tasha Agreste, Product Manager for the Proteomics product line, has been with CIL since 1989. During her tenure at CIL, Tasha has held various positions including customer service, sales and marketing, where she has proven to be instrumental in enhancing CIL’s customer relationships.

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