QC and Quantitation Kits
To help expedite quality control (QC) and quantitation in proteomics, CIL offers a number of off-the-shelf kits. In general, the QC kits are designed to evaluate the performance of the LC-MS platform, either alone or combination with the analytical workflow. The quantitation kits enable proteins to be measured in an absolute or relative manner using various bottom-up LC-MS methodologies and quantitation strategies. Each kit is described and referenced on their associated page.
Researchers in academia and life science industries continue to adopt a targeted, bottom-up MS-based proteomic workflow for biomarker discovery and validation. Biomarker validation requires absolute quantification of surrogate peptides in the sample matrix, a requirement that is best achieved using stable isotope-labeled standard (SIS) peptides. Read more.
The majority of research within the field of MS-based proteomics has focused on the relative quantitation of candidate protein biomarkers using bottom-up LC-MS methodologies in conjunction with stable isotope-labeled standards (SIS). Though significant progress has been made in the field of quantitative
proteomics, bottlenecks, such as assessing variability associated with proteolytic digestion and employing adequate quality control checks, often slow the process.