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MS/MS Standards

MS/MS Products

MS/MS Standards Catalog


  • MS/MS Standards Catalog
  • The Use of Stable Isotope-Enriched Standards as a Key Component of the MS/MS Analysis of Metabolites Extracted from Dried Blood Spots
Details and applications of our offerings (both sets and individuals) for MS/MS screening and tuning are noted in the resources and referencs below. In particular, the MS/MS Standards catalog lists the composition details and reconstitution recommendations (for concentrated and working stocks), but also the usage specifications (i.e., storage and recommended retest date before and after reconstitution) for our reference standard sets. The sets contain a collection of stable isotope-labeled standards (e.g., 12 amino acids in NSK-A) and are available in a 10-vial set or as single vials. CIL also has the ability to customize standards or mixes to meet your specific needs. Please inquire for details.
 

Resources

 

 

Frequently Asked Questions 

What is the recommended procedure for solubilizing NSK-A and -B?

The lyophilized amino acid mix in NSK-A (and NSK-A1) should  be dissolved in 1 mL of 50:50 purified water:methanol and the carnitine/acylcarnitines in NSK-B (and NSK-B-G1) in 1 mL of  purified methanol. Following the solvent addition, each vial should be vortexed manually for 1 min then auto-vortexed for 30 min (or longer until fully solubilized).

What’s the purpose of the NSK-AB-TS-2X1 tuning set?

These standards are used to tune the mass spectrometer and are to be measured independent of matrix samples and their preparation. These are intended to be processed before and after instrument maintenance, as well as batch analysis. The goal is to ensure that the MS/MS performance is optimal prior to precious sample analysis.

Are the component chemical purities considered in the NSK product formulations?

Yes, the chemical purity of each component (as stated on the CoA) is reviewed at time of formulation and the gravimetry is adjusted as necessary to compensate for chemical purity.

What concentration should I use on the CoA to represent the standard in use – gravimetric target concentration, concentration by gravimetry, or analyzed concentration?

The concentrations presented in the “Concentration by Gravimetry” column of the CoA should be used to represent the actual concentration of each component after reconstitution.

 

References

Coene, K.L.M.; Kluijtmans, L.A.J.; van der Heeft, E.; et al. 2018. Next-generation metabolic screening: targeted and untargeted metabolomics for the diagnosis of inborn errors of metabolism in individual patients. J Inherit Metab Dis, 41(3), 337-353. PMID: 29453510

Céspedes, N., Valencia, A., Echeverry, C.A., et al. 2017. Reference values of amino acids, acylcarnitines and succinylacetone by tandem mass spectrometry for use in newborn screening in southwest Colombia. Colomb Med, 48(3), 113-119. PMID: 29213153

de Sain-van der Velden, M.G.M.; van der Ham, M.; Gerrits, J.; et al. 2017. Quantification of metabolites in dried blood spots by direct infusion high resolution mass spectrometry. Anal Chim Acta, 979, 45-50. PMID: 28599708

Haynes, C.A.; De Jesús, V.R. 2016. Simultaneous quantitation of hexacosanoyl lysophosphatidylcholine, amino acids, acylcarnitines, and succinylacetone during FIA-ESI-MS/MS analysis of dried blood spot extracts for newborn screening. Clin Biochem, 49(1-2), 161-165. PMID: 26432925

Huang, T.; Cao, Y.; Zeng, J.; et al. 2016. Tandem mass spectrometry-based newborn screening strategy could be used to facilitate rapid and sensitive lung cancer diagnosis. Onco Targets Ther, 9, 2479-2487. PMID: 27217771

George, R.S.; Moat, S.J. 2016. Effect of dried blood spot quality on newborn screening analyte concentrations and recommendations for minimum acceptance criteria for sample analysis. Clin Chem, 62(3), 466-475. PMID: 26647314

Miller, M.J.; Kennedy, A.D.; Eckhart, A.D.; et al. 2015. Untargeted metabolomic analysis for the clinical screening of inborn errors of metabolism. J Inherit Metab Dis, 38(6), 1029-1039. PMID: 25875217

Held, P.K.; Haynes, C.A.; De Jesús, V.R.; et al. 2014. Development of an assay to simultaneously measure orotic acid, amino acids, and acylcarnitines in dried blood spots. Clin Chem Acta, 436, 149-154. PMID: 24886687

Chace, D.H.; Kalas, T.A.; Naylor, E.W. 2003. Use of tandem mass spectrometry for multianalyte screening of dried blood specimens from newborns. Clin Chem, 49, 1797-817. PMID: 14578311

Krista Backiel

Krista Backiel

Marketing Manager and Metabolomics Manager

Krista Backiel is responsible for managing and promoting products that are utilized in Metabolomics and Clinical/Diagnostic MS. She spends a lot of her time developing new products to assist customers in their diverse research efforts.

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Andrew Percy, PhD

Andrew Percy, PhD

Senior Applications Chemist – Mass Spectrometry

Dr. Andrew Percy is the Senior Applications Chemist for Mass Spectrometry. His responsibilities minimally involve overseeing product development, identifying new product market opportunities, assisting in the analysis of products for MS ‘omics applications, and providing technical support to customers.

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RELATED PRODUCTS

Carnitine/Acylcarnitine Standards
Amino Acid Standards
Substrates and Internal Standards
Organic Acids
Other Standards

 

To view information on our CE Mark* Products for MS/MS Standards click here.

*CE marking is a certification mark that indicates conformity with health, safety, and environmental protection standards for products sold within the European Economic Area
.