- Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT™) (Application Note 37)
- a 13C6-labeled and an unlabeled bi-phenyl reagent (4-phenethylbenzohydrazide, phenyl 2-GPN; for hydrazide tagging of free N-glycans)*,
- an unlabeled maltoheptaose oligosaccharide (for quality control of extraction, derivatization, and LC-MS), and
- a detailed instruction manual for performing the INLIGHT® strategy (see data file and data report below for maltodextrin, RNase B, and fetuin).
|INLIGHT® Product Information Sheet|
|Glycan Standards and N-Linked Glycan Quantitation Kit|
|INLIGHT® mzXML Data File (click here to download zip folder)|
|INLIGHT® Data Report|
Frequently Asked Questions
How many reactions can be performed with the INLIGHT® kit?
What is the representative dataset that is provided for the INLIGHT® kit?
The mzXML files contain the MS data obtained after applying the INLIGHT® strategy to maltodextrin (glycan), RNaseB (glycoprotein), and fetuin (glycoprotein). The corresponding “INLIGHT® Data Report” provides a summary of the glycans identified in these data files.
Can the INLIGHT® kit be used for O-glycan analysis?
No. The chemical cleavage of O-linked glycans does not leave a reducing terminus that is accessible to the INLIGHT® tagging reagent.
Mangrum, J.B.; Mehta, A.Y.; Alabbas, A.B.; Desai, U.R.; Hawkridge, A.M. 2017 Comparative analysis of INLIGHT™-labeled enzymatically depolymerized heparin by reverse-phase chromatography and high-performance mass spectrometry. Anal Bioanal Chem. PMID 27888308
Loziuk, PL; Hecht, ES; Muddiman, DC. 2016. N-linked glycosite profiling and use of Skyline as a platform for characterization and relative quantification of glycans in differentiating xylem of Populus trichocarpa. Anal Bioanal Chem. PMID: 27491298
Hecht, ES; McCord, JP; Muddiman, DC. 2016. A Quantitative Glycomics and Proteomics Combined Purification Strategy. J Vis Exp. 8;(109). PMID: 27023253
Hecht ES, Scholl EH, Walker SH, Taylor AD, Cliby WA, Motsinger-Reif AA, Muddiman DC. 2015. Relative Quantification and Higher-Order Modeling of the Plasma Glycan Cancer Burden Ratio in Ovarian Cancer Case-Control Samples. J Proteome Res. (10):4394-401. PMID: 26347193
Walker, S.H.; Taylor, A.D.; Muddiman, D.C. 2013. Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT): A Novel Glycan- Relative Quantification Strategy. J Am Soc Mass Spectrom, 24, 1376-1384. PMID: 23860851
Andrew Percy, PhD
Senior Applications Chemist - Mass Spectrometry
Dr. Andrew Percy is the Senior Applications Chemist for Mass Spectrometry. His responsibilities minimally involve overseeing product development, identifying new product market opportunities, assisting in the analysis of products for MS ‘omics applications, and providing technical support to customers.More
Proteomics Product Manager, Deuterated Reagents & Intermediates Product Manager, China Sales - Research Products
Tasha Agreste, Product Manager for the Proteomics product line, has been with CIL since 1989. During her tenure at CIL, Tasha has held various positions including customer service, sales and marketing, where she has proven to be instrumental in enhancing CIL’s customer relationships.More